MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data
MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data
复制标题
DOI：
10.1111/j.1471-8286.2004.00684.x
复制
发表时间：
2004-09-01
期刊：
影响因子：
--
通讯作者：
Shipley, P
中科院分区：
文献类型：
--
作者：
Van Oosterhout, C;Hutchinson, WF;Shipley, P
DNA降解、低DNA浓度和引物位点突变可能导致微卫星基因型的不正确分配，从而可能使群体遗传分析产生偏差。MICRO-BROCKER是一种基于WINDOWS（R）的软件，用于测试二倍体群体中微卫星的基因分型。该程序有助于识别由于非扩增等位基因（无效等位基因）、短等位基因优势（大等位基因缺失）和口吃峰评分导致的基因分型错误，并检测印刷错误。MICRO-BLOCKER估计无效等位基因的频率，并且重要的是，可以调整扩增等位基因的等位基因和基因型频率，允许它们用于进一步的群体遗传分析。
DNA degradation, low DNA concentrations and primer-site mutations may result in the incorrect assignment of microsatellite genotypes, potentially biasing population genetic analyses. MICRO-CHECKER is WINDOWS(R)-based software that tests the genotyping of microsatellites from diploid populations. The program aids identification of genotyping errors due to nonamplified alleles (null alleles), short allele dominance (large allele dropout) and the scoring of stutter peaks, and also detects typographic errors. MICRO-CHECKER estimates the frequency of null alleles and, importantly, can adjust the allele and genotype frequencies of the amplified alleles, permitting their use in further population genetic analysis.