Folding Paper-Based Aptasensor Platform Coated with Novel Nanoassemblies for Instant and Highly Sensitive Detection of 17β-Estradiol
Folding Paper-Based Aptasensor Platform Coated with Novel Nanoassemblies for Instant and Highly Sensitive Detection of 17β-Estradiol
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折叠式纸基适配体传感器平台涂有新型纳米组件，可即时、高灵敏地检测 17β-雌二醇
DOI：
10.1021/acssensors.9b01633
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发表时间：
2019-12-01
期刊：
影响因子：
8.9
通讯作者：
Cai, Xinxia
中科院分区：
文献类型：
--
作者：
Ming, Tao;Wang, Yang;Cai, Xinxia
由于其在女性生殖组织发育中的关键作用以及作为临床生物标志物，迫切需要开发一种快速且经济高效的方法来灵敏地检测 17 β-雌二醇 (E2)。在这项工作中，描述了一种具有微流体通道的折叠适体传感器平台，用于 E2 的无标记电化学检测。该平台采用精巧的折叠结构设计，集过滤孔、微流通道、反应室和三电极系统于一体，使用起来非常方便。为了提高检测灵敏度并固定适体，我们合成了一种由胺功能化单壁碳纳米管/新型亚甲蓝/金纳米颗粒（AuNP）组成的新型纳米组件，并用该纳米组件修饰了工作电极。实验结果获得的校准曲线在10 pg mL(-1)至500 ng mL(-1)之间呈现线性范围(R-2 = 0.993)，检测限达到5 pg mL-1(S/N = 3)。此外，还进行了临床血清中E2的检测实验，结果与使用大型电化学发光装置获得的结果高度相似。通过集成多个功能组件、采用新颖的纳米组件和折叠结构，该纸基平台不仅具有作为E2即时检测的简单便捷的集成装置的巨大潜力，而且作为便携式、低成本、高灵敏度的适体传感器平台，能够利用合适的适体检测多种诊断生物标志物。
Owing to its critical role in the development of female reproductive tissues and as a clinical biomarker, there is an urgent need to develop a rapid and cost-effective method to sensitively detect 17 beta-estradiol (E2). In this work, a folding aptasensor platform with microfluidic channels for the label-free electrochemical detection of E2 is described. The platform, designed with a delicate folding structure, integrating filter holes, microfluidic channels, reaction chambers, and a three electrode system, is extremely easy to use. To increase the detection sensitivity and immobilize the aptamer, we synthesized a novel nanoassembly consisting of amine-functionalized single-walled carbon nanotube/new methylene blue/gold nanoparticles (AuNPs) and modified the working electrode with this nanoassembly. The calibration curve obtained from the experimental results exhibited a linear range between 10 pg mL(-1) and 500 ng mL(-1) (R-2 = 0.993), and a detection limit of 5 pg mL-1 was achieved (S/N = 3). Furthermore, experiments to detect E2 in clinical serum were conducted, and the results were highly similar to those obtained using a large electrochemical luminescence apparatus. By integrating multiple functional components, adopting novel nanoassemblies, and using a folding structure, this paper-based platform not only has great potential as a simple and convenient integrated device for point-of-care testing of E2, but also as a portable, low-cost, and highly sensitive aptasensor platform capable of detecting many diagnostic biomarkers with the appropriate aptamers.