Parallel Use of Two Behavioral Mechanisms for Chemotaxis in Caenorhabditis elegans
Parallel Use of Two Behavioral Mechanisms for Chemotaxis in Caenorhabditis elegans
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DOI：
10.1523/jneurosci.3633-08.2009
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发表时间：
2009-04-29
影响因子：
5.3
通讯作者：
Yoshida, Kazushi
中科院分区：
文献类型：
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作者：
Iino, Yuichi;Yoshida, Kazushi
秀丽隐杆线虫对各种气味物质和水溶性化学引诱物如NaCl具有趋化性。以前的研究描述了趋化性的旋转机制，其中C。当化学物质的浓度降低时，秀丽线虫会通过一系列固定的行为，如脚尖旋转，迅速改变运动方向。在这里，我们报告发现了第二种趋化机制，称为风向标机制。在这种策略中，动物对化学引诱物的空间梯度做出反应，并逐渐向更高浓度的化学物质弯曲。通过计算机模拟，我们发现这两种机制都有助于趋化性，这两种机制需要并行有效的趋化性。使用激光消融单个神经元检查潜在的神经回路，我们发现ASE感觉神经元和AIZ中间神经元是必不可少的pirouette和风向标机制的趋化性NaCl。盐适应的动物在这两种行为中表现出相反的反应，导致避免NaCl。这些结果提供了一个平台，详细的分子和细胞分析的趋化性和可塑性，在这个模式生物。
Caenorhabditis elegans shows chemotaxis to various odorants and water-soluble chemoattractants such as NaCl. Previous studies described the pirouette mechanism for chemotaxis, in which C. elegans quickly changes the direction of locomotion by using a set of stereotyped behaviors, a pirouette, in response to a decrease in the concentration of the chemical. Here, we report the discovery of a second mechanism for chemotaxis, called the weathervane mechanism. In this strategy animals respond to a spatial gradient of chemoattractant and gradually curve toward higher concentration of the chemical. By computer simulation, we find that both of these mechanisms contribute to chemotaxis and both mechanisms need to act in parallel for efficient chemotaxis. Using laser ablation of individual neurons to examine the underlying neural circuit, we find the ASE sensory neurons and AIZ interneurons are essential for both the pirouette and weathervane mechanisms in chemotaxis to NaCl. Salt-conditioned animals show reversed responses in both of these behaviors, leading to avoidance of NaCl. These results provide a platform for detailed molecular and cellular analyses of chemotaxis and its plasticity in this model organism.