Antibacterial Nanocomposite with Calcium Phosphate and Quaternary Ammonium
Antibacterial Nanocomposite with Calcium Phosphate and Quaternary Ammonium
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DOI：
10.1177/0022034512440579
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发表时间：
2012-05-01
影响因子：
7.6
通讯作者：
Xu, H. H. K.
中科院分区：
文献类型：
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作者：
Cheng, L.;Weir, M. D.;Xu, H. H. K.
继发性龋病是修复失败的常见原因，主要由产酸细菌及其生物膜引起。本研究的目的是：(1)研制一种新型的含有无定形磷酸钙(NACP)和季铵盐二甲基丙烯酸酯(QADM)纳米颗粒的纳米复合材料；(2)研究其力学性能和抗菌性能。采用喷雾干燥法制备了粒径为116 nm的NACP。该纳米复合材料含有NACP和增强玻璃填料，树脂中含有QADM。两种商用复合材料作为对照进行了测试。将变形链球菌接种到复合材料中。经过180天的水老化后，NACP+QADM纳米复合材料的弯曲强度和弹性模量达到了商业对照的水平(p>；0.1)。与商用复合材料(p<；0.05)相比，NACP+QADM纳米复合材料的生物被膜集落形成单位(CFU)减少了3倍。NACP+QADM生物膜的代谢活性和乳酸产量明显低于商品复合材料(p<；0.05)。水老化30、90和180d后，NACP+QADM的抗菌性能保持不变(p>；0.05)。总之，新型的NACP-QADM纳米复合材料大大降低了生物被膜的代谢活性、CFU和乳酸，同时与没有抗菌性能的商业复合材料的承载能力相当。NACP-QADM纳米复合材料具有强大和持久的抗菌性能，加上之前报道的Ca-PO4释放能力，可能会使其用于防龋性修复。
Secondary caries is a frequent reason for restoration failure, resulting from acidogenic bacteria and their biofilms. The objectives of this study were to: (1) develop a novel nanocomposite containing nanoparticles of amorphous calcium phosphate (NACP) and quaternary ammonium dimethacrylate (QADM); and (2) investigate its mechanical and antibacterial durability. A spray-drying technique yielded NACP with particle size of 116 nm. The nanocomposite contained NACP and reinforcement glass fillers, with QADM in the resin. Two commercial composites were tested as controls. Composites were inoculated with Streptococcus mutans. After 180-day water-aging, NACP+QADM nanocomposite had flexural strength and elastic modulus matching those of commercial controls (p > 0.1). NACP+QADM nanocomposite reduced the biofilm colony-forming units (CFU) by 3-fold, compared with commercial composites (p < 0.05). Metabolic activity and lactic acid production of biofilms on NACP+QADM were much less than those on commercial composites (p < 0.05). The antibacterial properties of NACP+QADM were maintained after water-aging for 30, 90, and 180 d (p > 0.05). In conclusion, the novel NACP-QADM nanocomposite greatly decreased biofilm metabolic activity, CFU, and lactic acid, while matching the load-bearing capability of commercial composites without antibacterial properties. The NACP-QADM nanocomposite with strong and durable antibacterial properties, together with its previously reported Ca-PO4 release capability, may render it useful for caries-inhibiting restorations.