A simplified procedure for the isolation of the sucrase-isomaltase complex from rabbit intestine. Its amino-acid and sugar composition.
A simplified procedure for the isolation of the sucrase-isomaltase complex from rabbit intestine. Its amino-acid and sugar composition.
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从兔肠中分离蔗糖酶-异麦芽酶复合物的简化程序。
DOI：
10.1111/j.1432-1033.1972.tb02065.x
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发表时间：
1972
期刊：
影响因子：
--
通讯作者：
G. Semenza
中科院分区：
文献类型：
--
作者：
A. Cogoli;H. Mosimann;C. Vock;A. K. von Balthazar;G. Semenza
本文提出了一种从兔小肠中分离蔗糖酶·异麦芽糖酶复合物的简便方法，该方法能以较好的产率以均一的形式制备该蛋白。
兔小肠蔗糖酶·异麦芽糖酶复合物是一种糖蛋白。它富含酸性氨基酸;在天然状态下没有游离巯基，但变性会暴露六个巯基。酪氨酸/色氨酸比例为2。糖部分（150%）由d-葡萄糖、d-半乳糖、d-甘露糖、岩藻糖、葡糖胺和半乳糖胺（后两者可能是乙酰化的）组成。蔗糖酶·异麦芽糖酶复合物不含神经氨酸、糖醛酸、脂质、磷酸盐、硫酸盐或锌。
从氨基酸和糖组成计算的偏比容与实验测定的偏比容一致。这种膜蛋白的疏水系数与其他膜结合蛋白或水溶性蛋白没有区别。
A simplified procedure for the isolation of the sucrase · isomaltase complex from rabbit small intestine was worked out, which allows the preparation of this protein in a homogeneous form in good yield.
The sucrase · isomaltase complex from rabbit small intestine is a glycoprotein. It is rich in acidic amino acids; it has no free thiols in the native state but denaturation unmasks six thiols. The tyrosine/tryptophan ratio is 2. The sugar moiety(ies) (150%) is composed of d-glucose, d-galactose, d-mannose, fucose, glucosamine and galactosamine (the latter two are probably acetylated). The sucrase · isomaltase complex contains no neuraminic, uronic acid, lipids, phosphate, sulfate or zinc.
The partial specific volume, calculated from the amino acid and sugar composition, agrees with that experimentally determined. The hydrophobicity coefficient of this membrane protein does not differ from that of other membrane bound or water soluble proteins.