Interaction of Neisseria maningitidis with the components of the blood-brain barrier correlates with an increased expression of PilC.
Interaction of Neisseria maningitidis with the components of the blood-brain barrier correlates with an increased expression of PilC.
复制标题
脑膜炎奈瑟菌与血脑屏障成分的相互作用与 PilC 表达的增加相关。
DOI：
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发表时间：
1997
影响因子：
6.4
通讯作者：
Xavier Nassif
中科院分区：
文献类型：
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作者：
Bénédicte Pron;M. Taha;Caroline Rambaud;J.;Natacha Pattey;Jean;M. Musílek;J. Beretti;Xavier Nassif
对一例未经治疗的致命性暴发性脑膜炎球菌血症病例进行了检查，以调查脑膜炎奈瑟菌是否穿过血脑屏障（BBB）。显微镜检查显示体内细菌粘附在脉络丛和脑膜的内皮上。对从血液和脑脊液 (CSF) 中培养的分离株进行比较，发现菌毛蛋白或 5 类蛋白没有抗原变异，而 PilC 蛋白在 CSF 和脉络丛中的表达量高于血液中的表达量。这是由于 pilC 启动子之一的活性增加所致。 PilC 的较高表达在体外与内皮细胞的较高粘附性相关。该菌株的单个 pilC 位点的突变消除了体外菌毛介导的内皮细胞粘附。这些数据表明，PilC 在 BBB 的穿越中发挥着重要作用，可能是通过菌毛介导的粘附。
A fatal untreated case of fulminant meningococcemia was examined to investigate the crossing of the blood-brain barrier (BBB) by Neisseria meningitidis. Microscopic examination showed bacteria in vivo adhering to the endothelium of both the choroid plexus and the meninges. Comparison of the isolates cultivated from the blood and the cerebrospinal fluid (CSF) revealed no antigenic variation of the pilin or the class 5 protein, whereas the expression of the PilC protein was greater in the CSF and the choroid plexus than in the blood. This was due to an increased activity of one of the pilC promotors. This higher expression of PilC correlated in vitro with greater adhesiveness to endothelial cells. A mutation in the single pilC locus of this strain abolished in vitro pilus-mediated adhesion to endothelial cells. These data suggest that PilC plays an important role in the crossing of the BBB, likely through pilus-mediated adhesion.