Acute endocrine and nutritional co-regulation of the hepatic omy-miRNA-122b and the lipogenic gene fas in rainbow trout, Oncorhynchus mykiss
Acute endocrine and nutritional co-regulation of the hepatic omy-miRNA-122b and the lipogenic gene fas in rainbow trout, Oncorhynchus mykiss
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DOI：
10.1016/j.cbpb.2013.12.002
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发表时间：
2014-03-01
影响因子：
2.2
通讯作者：
Skiba-Cassy, Sandrine
中科院分区：
文献类型：
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作者：
Mennigen, Jan A.;Plagnes-Juan, Elisabeth;Skiba-Cassy, Sandrine
肝脏脂肪生成是中间代谢的重要组成部分，并受到内分泌因子和营养素的强烈调节。肝脏特异性和高度丰富的microRNA-122已成为高等脊椎动物脂肪生成的强大调节因子，但对其内分泌和营养调节知之甚少。在这项研究中，我们研究了胰岛素调节肝脏表达的omy-miRNA-122 isomiRNA（omy-miRNA-122 a和omy-miRNA-122 b）的假设，通过使用体内和体外方法。由于虹鳟鱼的肝脏胰岛素途径和脂肪生成受到饮食中大量营养素比例的剧烈调节，我们进一步研究了改变碳水化合物/蛋白质比例和脂质/蛋白质比例的单餐对omy-miRNA-122 isomiRNAs餐后表达的影响。胰岛素在体内和体外急性诱导omy-miRNA-122 b表达。相反，增加脂质与蛋白质比例的单餐急剧降低了两种my-miRNA-122 isomiRNA的表达。作为miRNA-122在鱼类中的脂肪生成作用的直接证据，我们研究了my-miRNA-122 isomiRNA与哺乳动物中miRNA-122的间接靶基因fas的相关表达。我们的研究结果表明，omy-miRNA-122 b和Fas的显着正相关性，与潜在的进化保守的作用，miRNA-122在鳟鱼餐后脂肪生成的调节一致。(C)版权所有© 2014 Elsevier Inc.
Hepatic lipogenesis represents a crucial part of intermediary metabolism and is acutely regulated by endocrine factors and nutrients. The liver-specific and highly abundant microRNA-122 has emerged as a powerful regulator of lipogenesis in higher vertebrates, but little is known about its endocrine and nutritional regulation. In this study, we investigated the hypothesis that insulin regulates hepatic expression of omy-miRNA-122 isomiRNAs (omy-miRNA-122a and omy-miRNA-122b) by using in vivo and in vitro approaches. Since the hepatic insulin pathway and lipogenesis are acutely regulated by dietary macronutrient ratios in rainbow trout, we further investigated the effect of single meals with altered carbohydrate/protein ratio and lipid/protein ratio on the postprandial expression of omy-miRNA-122 isomiRNAs. Insulin acutely induced omy-miRNA-122b expression in vivo and in vitro. Conversely, a single meal with increased lipid to protein ratio acutely decreased expression of both omy-miRNA-122 isomiRNAs. As a direct proof of lipogenic effects of miRNA-122 is currently still lacking in fish, we investigated the correlated expression between omy-miRNA-122 isomiRNAs and the rate-limiting lipogenic gene fas, an indirect target gene of miRNA-122 in mammals. Our results show a significant positive correlation of omy-miRNA-122b and fas, consistent with a potential evolutionary conserved role for miRNA-122 in the regulation of postprandial lipogenesis in trout. (C) 2014 Elsevier Inc All rights reserved.