Prenatal exposure to bisphenol-A is associated with Toll-like receptor-induced cytokine suppression in neonates
Prenatal exposure to bisphenol-A is associated with Toll-like receptor-induced cytokine suppression in neonates
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DOI：
10.1038/pr.2015.234
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发表时间：
2016-03-01
影响因子：
3.6
通讯作者：
Huang, Jing-Long
中科院分区：
文献类型：
--
作者：
Liao, Sui-Ling;Tsai, Ming-Han;Huang, Jing-Long
背景：尽管人类广泛暴露于双酚A(BPA)，但关于BPA与幼儿不良健康结局之间的关系的研究有限。本研究旨在探讨产前暴露于双酚A对新生儿Toll样受体诱导的细胞因子反应的影响及其与日后感染疾病的关系。方法：采集275例足月新生儿脐带血。用Toll样受体配体(TLR1-4和7-8)刺激单个核细胞，检测其产生的肿瘤坏死因子-α、白介素6和白介素10。采用双抗体夹心酶联免疫吸附分析法检测血清BPA浓度。结果：脐血BPA浓度与TLR3和TLR4刺激的肿瘤坏死因子-α反应(P=0.001.0 5)和TLR78刺激的IL-6反应(P=0.0 3)显著相关。临床分析未显示产前双酚A暴露与出生第一年的感染或细菌定植有关。结论：这是首次队列研究表明产前双酚A暴露在新生儿TLR相关的先天免疫反应中起作用。然而，尽管免疫平衡发生了变化，结果并未显示这种暴露与婴儿早期感染风险的增加有关。
Background: Despite widespread human exposure to biphenol A (BPA), limited studies exist on the association of BPA with adverse health outcomes in young children. This study aims to investigate the effect of prenatal exposure to BPA on toll-like receptor-induced cytokine responses in neonates and its association with infectious diseases later in life.Methods: Cord bloods were collected from 275 full-term neonates. Production of TNF-alpha, IL-6, and IL-10 were evaluated after stimulating mononuclear cells with toll-like receptor ligands (TLR1-4 and 7-8). Serum BPA concentrations were analyzed by enzyme-linked immunosorbent assay. Bacteria from nasopharyngeal specimens were identified with multiplex PCR and culture method.Result: Result showed significant association between cord BPA concentration and TLR3- and TLR4-stimulated TNF-a response (P = 0.001) and that of TLR78-stimulated IL-6 response (P = 0.03). Clinical analysis did not show prenatal BPA exposure to be correlated with infection or bacterial colonization during the first year of life.Conclusion: This is the first cohort study that indicated prenatal BPA exposure to play a part in TLR-related innate immune response of neonatal infants. However, despite an altered immune homeostasis, result did not show such exposure to be associated with increased risk of infection during early infancy.