Integrin repertoire on myogenic cells changes during the course of primary myogenesis in the mouse
Integrin repertoire on myogenic cells changes during the course of primary myogenesis in the mouse
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DOI：
10.1002/dvdy.20280
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发表时间：
2005-04-01
影响因子：
2.5
通讯作者：
Thorsteinsdóttir, S
中科院分区：
文献类型：
--
作者：
Cachaço, AS;Pereira, CS;Thorsteinsdóttir, S
细胞通过受体与细胞外基质相互作用，最常见的是整合素家族。我们(cachaco等人)[20031发展130：1659年-1671年]和其他(施万德等人)。[20031开发细胞4：673-685)已经证明了β1整合素在小鼠初级肌肉发生中的作用。然而，目前还不清楚在体内的这一过程中，有什么亚基与β1配对。在这里，我们确定了一个亚基在胚胎(E)11.5-E14.5天的表达模式。分化的肌瘤细胞表达所研究的所有A.亚基。由于肌块形成在躯干(E12.5)和四肢(E11.5-E12.5)，层粘连蛋白受体α6β1和α7β1是检测不到的，并且缺乏组装的层粘连蛋白基质。相反，表达α1β1、α4β1、α5β1和含有αv的整合素，未组装的层粘连蛋白和纤维连接蛋白在肌源性细胞周围大量存在。在E13.5-E14.5，表达α6β1和α7β1，并在单个肌管周围形成层粘连蛋白基质。因此，在小鼠的原代肌肉发生过程中，肌源性细胞改变了它们的整合素表达模式，这表明纤维连接蛋白和层粘连蛋白基质在这一过程中扮演着不同的角色。(C)2005年Wiley-Liss，Inc.
Cells interact with the extracellular matrix through receptors, most commonly of the integrin family. We (Cachaco et al. [20031 Development 130:1659-1671) and others (Schwander et al. [20031 Dev. Cell 4:673-685) have demonstrated a role for beta 1 integrins in mouse primary myogenesis. However, it is unclear what a subunits pair with beta 1 during this process in vivo. Here, we determined a subunit expression patterns at embryonic day (E) 11.5-E14.5. Differentiated myotomal myocytes express all a. subunits studied. As the muscle masses form both in trunk (E12.5) and limbs (E11.5-E12.5), laminin receptors alpha 6 beta 1 and alpha 7 beta 1 are undetectable, and an assembled laminin matrix is absent. Instead alpha 1 beta 1, alpha 4 beta 1, alpha 5 beta 1 and an alpha v-containing integrin are expressed and unassembled laminin and fibronectin are abundant around myogenic cells. At E13.5-E14.5, alpha 6 beta 1 and alpha 7 beta 1 are expressed, and a laminin matrix forms around individual myotubes. Thus, myogenic cells change their integrin expression pattern during the course of primary myogenesis in the mouse, suggesting different roles for fibronectin- and laminin-containing matrices in this process. (c) 2005 Wiley-Liss, Inc.