Histological and scanning electron microscopy analyses of bone/implant interface using the novel bonelikee synthetic bone graft
Histological and scanning electron microscopy analyses of bone/implant interface using the novel bonelikee synthetic bone graft
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DOI：
10.1002/jor.20117
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发表时间：
2006-05-01
影响因子：
2.8
通讯作者：
Santos, JD
中科院分区：
文献类型：
--
作者：
Gutierres, M;Hussain, NS;Santos, JD
合成骨移植物提供了自体移植物和同种异体移植物的替代方案。类骨（R）是一种专利的合成骨移植物，模仿骨的矿物成分。本研究的目的是在将该材料用于骨再生的骨科应用之前，评估Bonelike（R）（g）移植物在人体中的生物学行为，例如，用于内侧膝关节骨关节炎的开放楔形高胫骨截骨术。将类骨颗粒植入11例骨缺损患者的皮质骨中，分析其新骨形成、骨传导特性和骨吸收特性。颗粒范围为500至1000 μ m，植入胫骨外侧。患者的平均年龄为59岁（范围48至70岁）;有8名女性和3名男性，均患有膝关节内侧间室骨关节炎。手术时，在螺钉进入点远端3 cm处创建1 x 1 x 1 cm皮质缺损，与胫骨长轴一致。在植入6、9和12个月后取出金属假体期间，提取植入的Bonelike（R）移植物样本进行分析。放射学随访。对回收的样品进行扫描电子显微镜、组织学分析和组织形态测量，以评估缺损区域的骨再生。通过植入颗粒周围广泛的成熟骨形成和高水平的骨-移植物接触百分比（67-84%）证明了骨传导能力。Bonelike（R）作为一种优良的生物活性支架，允许骨细胞在其表面上迁移、增殖和分化，因此以快速、可控的方式实现了缺损的再生。我们的研究结果表明，类骨（R）移植物是一个很好的候选人，骨科应用中快速新骨形成是一个基本要求。(c)2006骨科研究学会。出版社：Wiley Periodicals，Inc. J Orthop Res 24：953-958，2006.
Synthetic bone grafts provide an alternative to autografts and allografts. Bonelike (R) is a patented synthetic bone graft that mimics the mineral composition of bone. The aim of the present work was to assess the biological behavior of Bonelike (R)(g) graft in humans, before using the material in orthopedic applications of bone regeneration, for example, in opening wedge high tibial osteotornies for medial knee osteoarthritis. Bonelike (R) granules were implanted in cortical bone of 11 patients undergoing osteotornies, and new bone formation, osteoconductive proper-ties, and resorption characteristics of the granules were analyzed. The granules ranged from 500 to 1000 mu m and were implanted in the lateral aspect of the tibia. The patients' mean age was 59 years (range 48 to 70 years); there were eight women and three men, all suffering from medial compartment osteoarthritis of the knee. At surgery, a 1 x 1 x 1-cm cortical defect was created 3 cm distal to the entry point of the screws, in line with the long axis of tibia. The implanted Bonelike (R) graft sample was extracted for analysis during removal of the metallic prosthesis after implantation times of 6, 9, and 12 months. Radiological follow-up,. scanning electron microscopy, histological analysis, and histomorphometric measurements were conducted on the retrieved samples to assess bone regeneration in the defect area. Osteoconductive capacity was demonstrated by extensive mature bone formation around the implanted granules and high levels of percentage bone-to-graft contact (from 67-84%). Bonelike (R) acted as an excellent bioactive scaffold, allowing the migration, proliferation, and differentiation of bone cells on its surface, and therefore regeneration of the defects was achieved in a rapid, controlled manner. Our results suggest that Bonelike (R) graft is an excellent candidate for orthopedic applications where rapid new bone formation is a fundamental requirement. (c) 2006 Orthopaeclic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 24:953-958, 2006.