Mannheimia haemolytica chimeric protein vaccine composed of the major surface-exposed epitope of outer membrane lipoprotein PlpE and the neutralizing epitope of leukotoxin
Mannheimia haemolytica chimeric protein vaccine composed of the major surface-exposed epitope of outer membrane lipoprotein PlpE and the neutralizing epitope of leukotoxin
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DOI：
10.1016/j.vaccine.2008.07.023
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发表时间：
2008-09-08
期刊：
影响因子：
5.5
通讯作者：
Taylor, Jared D.
中科院分区：
文献类型：
--
作者：
Ayalew, Sahlu;Confer, Anthony W.;Taylor, Jared D.
溶血性曼氏菌通常在患有运输热肺炎的牛中发现。目前可用的疫苗不能完全预防这种疾病。为了开发递送白细胞毒素（LKT）A和外膜蛋白（OMP）PlpE的免疫原性区域的疫苗，构建了总共四种嵌合蛋白。用25、50和75 μ g量的每种嵌合蛋白皮下免疫小鼠。免疫反应的特异性通过Western印迹分析和酶联免疫吸附测定（ELISA）证实。超免疫血清对M.溶血性补体和中和LKT的存在下。虽然所有的嵌合蛋白都诱导了一定水平的免疫应答，但其中两种，SAC 87和SAC 89，是最有希望的。这些结果表明，针对M.溶血性链球菌可以通过用从LKT和PlpE蛋白的特异性免疫原性区域产生的重组嵌合蛋白接种来诱导。
Mannheimia haemolytica is commonly identified in cattle with shipping fever pneumonia. Vaccines currently available do not provide complete protection against the disease. In an effort to develop a vaccine that delivers the immunogenic regions of leukotoxin (LKT) A and the outer membrane protein (OMP) PlpE, a total of four chimeric proteins were constructed. Mice were subcutaneously immunized with 25, 50 and 75 mu g quantities of each chimeric protein. The specificity of the immune response was confirmed by Western blot analysis and enzyme-linked immunosorbent assays (ELISA). Moreover, the hyperimmune sera were bactericidal to M. haemolytica in the presence of complement and neutralized LKT. While all of the chimeric proteins induced some level of immune response two, SAC87 and SAC89, were most promising. These results demonstrate that a functional immune response against M. haemolytica can be induced by vaccination with recombinant chimeric proteins created from specific immunogenic regions of the LKT and PlpE proteins.