flaA mRNA transcription level correlates with Helicobacter pylori colonisation efficiency in gnotobiotic piglets.
flaA mRNA transcription level correlates with Helicobacter pylori colonisation efficiency in gnotobiotic piglets.
复制标题
flaA mRNA 转录水平与幽门螺杆菌在无菌仔猪中的定植效率相关。
DOI：
10.1099/00222615-48-4-395
复制
发表时间：
1999
影响因子：
3
通讯作者：
Eaton,KA
中科院分区：
文献类型：
--
作者：
Mankoski,R;Hoepf,T;Krakowka,S;Eaton,KA
In-vivo passage of the gastric pathogenHelicobacter pyloriin gnotobiotic piglets results in a greater colonisation efficiency in subsequent infections. The rate of colonisation steadily increases with the number of passages. To determine if this increased efficiency is related to the level of expression offlaA, a gene which encodes the major subunit of flagella, this study evaluated the level offlaAexpression at five points during serial in-vivo passage of strain 26695. Semi-quantitative reverse transcriptase polymerase chain reaction and Northern dot-blot analysis offlaAmRNA levels (expressed as a ratio to the level ofureAmRNA) revealed a positive correlation betweenflaAexpression and colonisation efficiency;flaAmRNA levels increased incrementally with in-vivo passage as did colonisation rates. Immunoblots of outer-membrane vesicles from pig-passaged and laboratory-passaged strains also demonstrated marked differences in the amount of flagellin present in poor and efficient colonisers.
