Quantitative Profiling of Arabidopsis Polar Glycerolipids under Two Types of Heat Stress
Quantitative Profiling of Arabidopsis Polar Glycerolipids under Two Types of Heat Stress
复制标题
两种热应激下拟南芥极性甘油脂的定量分析
DOI：
10.3390/plants9060693
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发表时间：
2020-06-01
期刊：
影响因子：
4.5
通讯作者：
Yu, Buzhu
中科院分区：
文献类型：
--
作者：
Qin, Feng;Lin, Liang;Yu, Buzhu
在细胞水平上，膜脂重塑和热休克蛋白的产生是植物在热胁迫下生存的两个主要策略。虽然许多关于甘油脂和热休克蛋白的研究已经被单独报道，但甘油脂的详细变化以及热休克蛋白在甘油脂变化中的作用仍有待揭示。在这项研究中，我们描述了两种类型的热胁迫下野生型拟南芥及其HSP101缺陷型突变体-1的甘油脂。我们的结果表明，在热胁迫过程中，野生型拟南芥和HOT-1甘油脂的变化非常相似。虽然热驯化导致甘油脂略有下降，但未经热驯化的植物在热激条件下甘油脂的下降更为严重。中度热胁迫下，36：X单半乳糖二酰甘油(MGDG)含量略有增加，34：6 MGDG和34：4磷脂酰甘油(PG)含量严重下降。我们的研究结果表明，热习服可以减少甘油脂在热休克下的降解。在中等温度胁迫下，原核途径甘油脂合成受到严重抑制，而真核途径甘油脂合成略有增强。此外，热休克蛋白101对热应激下甘油三脂的变化影响不大。
At the cellular level, the remodelling of membrane lipids and production of heat shock proteins are the two main strategies whereby plants survive heat stress. Although many studies related to glycerolipids and HSPs under heat stress have been reported separately, detailed alterations of glycerolipids and the role of HSPs in the alterations of glycerolipids still need to be revealed. In this study, we profiled the glycerolipids of wild-type Arabidopsis and its HSP101-deficient mutanthot-1 under two types of heat stress. Our results demonstrated that the alterations of glycerolipids were very similar in wild-type Arabidopsis andhot-1during heat stress. Although heat acclimation led to a slight decrease of glycerolipids, the decrease of glycerolipids in plants without heat acclimation is more severe under heat shock. The contents of 36:x monogalactosyl diacylglycerol (MGDG) were slightly increased, whereas that of 34:6 MGDG and 34:4 phosphatidylglycerol (PG) were severely decreased during moderate heat stress. Our findings suggested that heat acclimation could reduce the degradation of glycerolipids under heat shock. Synthesis of glycerolipids through the prokaryotic pathway was severely suppressed, whereas that through the eukaryotic pathway was slightly enhanced during moderate heat stress. In addition, HSP101 has a minor effect on the alterations of glycerolipids under heat stress.