Identification of two types of specific endothelin receptors in rat mesangial cell.
Identification of two types of specific endothelin receptors in rat mesangial cell.
复制标题
大鼠系膜细胞中两类特异性内皮素受体的鉴定。
DOI：
10.1016/0006-291x(89)90829-2
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发表时间：
1989
影响因子：
3.1
通讯作者：
Inagami,T
中科院分区：
文献类型：
--
作者：
Sugiura,M;Snajdar,RM;Schwartzberg,M;Badr,KF;Inagami,T
用交联法在体外培养的大鼠肾小球系膜细胞上鉴定出两种类型的内皮素受体。在十二烷基硫酸钠存在下，经聚丙烯酰胺凝胶电泳法测定，这些受体的相对分子质量分别约为58,000和34,000。硝苯地平、尼卡地平、维拉帕米、地尔硫卓、血管紧张素II或[Arg8]-血管加压素不能抑制放射性碘标记内皮素与其受体的结合。内皮素结合蛋白用1%洋地黄素溶解，在非变性条件下用凝胶过滤进行分级。在相对分子质量为65,000和43,000的位置有两个内皮素结合峰。这些观察结果表明，大鼠肾小球系膜细胞存在两种不同于电压依赖性L型钙通道的特异性内皮素受体。
Two types of receptors specific for endothelin were identified using cross-linking technique in cultured rat mesangial cells. The molecular weights of these receptors were approximately 58,000 and 34,000 by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The binding of radio-iodinated-endothelin to its receptors was inhibited by excess of unlabeled endothelin, but not by nifedipine, nicardipine, verapamil, diltiazem, angiotensin II or [Arg8]-vasopressin. The endothelin binding proteins were solubilized with 1% digitonin and fractionated under non-denaturing conditions by gel filtration. Two endothelin binding peaks eluted at the positions corresponding to the molecular weights of 65,000 and 43,000. These observations indicate that there are two types of specific endothelin receptors in rat mesangial cells which are distinct from voltage dependent L-type calcium channel.