Cationic Proteins of Polymorphonuclear Leukocyte Lysosomes II. Composition, Properties, and Mechanism of Antibacterial Action
Cationic Proteins of Polymorphonuclear Leukocyte Lysosomes II. Composition, Properties, and Mechanism of Antibacterial Action
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多形核白细胞溶酶体的阳离子蛋白 II。
DOI：
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发表时间：
1966
影响因子：
3.2
通讯作者：
J. Spitznagel
中科院分区：
文献类型：
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作者：
H. I. Zeya;J. Spitznagel
泽亚，H. I.（北卡罗来纳州，查佩尔山大学）和J. K。斯皮茨纳格尔多形核白细胞溶酶体的阳离子蛋白质。二.抗菌作用的组成、性质和机制。J. Bacteriol. 91：755-762。1966.-豚鼠多形核细胞（PMN）颗粒经酸提、20%（v/v）乙醇沉淀后，得到一种碱性蛋白组分。该馏分占大部分的PMN颗粒的抗菌活性，并对应于抗菌阳离子成分的完整的颗粒（带I，II和III），通过区带电泳解决。与完整溶酶体的酶组分相同的组分的级分的缺失表明该级分基本上不含溶酶体酶。蛋白质的氨基酸分析表明，碱性氨基酸占优势（25%），特别是精氨酸（16%）。比较氨基酸分析表明，溶酶体阳离子蛋白（LCP）级分是显着不同的核组蛋白。LCP馏分表现出对某些革兰氏阳性菌和革兰氏阳性微生物，包括白色念珠菌的抗菌活性，并表现出其活性的化学计量关系。用LCP组分处理的微生物凝集。核酸、肝素和内毒素等阴离子物质有效地阻断了该组分的抗菌活性。LCP组分抑制细菌细胞的氧摄取，并破坏细胞的渗透屏障，表现为上清液中P32以及260 mμ处的紫外线吸收材料的快速释放。
Zeya, H. I. (University of North Carolina, Chapel Hill), and J. K. Spitznagel. Cationic proteins of polymorphonuclear leukocyte lysosomes. II. Composition, properties, and mechanism of antibacterial action. J. Bacteriol. 91:755–762. 1966.—A basic proteins fraction from guinea pig polymorphonuclear (PMN) granules was obtained by acid extraction and precipitation with 20% (v/v) ethyl alcohol. The fraction accounted for most of the antibacterial activity of the PMN granules and corresponded to the antibacterial cationic components of intact granules (bands I, II, and III) resolved by zone electrophoresis. Absence from the fraction of components identical to the enzymatic components of intact lysosomes showed that the fraction was essentially free from lysosomal enzymes. The amino acid analysis of proteins in the fraction gave a preponderance of basic amino acids (25%), especially of arginine (16%). The comparative amino acid analysis showed that the lysosomal cationic proteins (LCP) fraction was markedly different from nuclear histones. The LCP fraction manifested antibacterial activity against certain gram-positive and gram-positive microorganisms, including Candida albicans, and exhibited stoichiometric relationship in its activity. Microorganisms treated with LCP fraction were agglutinated. Anionic substances such as nucleic acids, heparin, and endotoxin effectively blocked the antibacterial activity of the fraction. The LCP fraction caused suppression of oxygen uptake by bacterial cells and damaged the permeability barriers of cells as manifested by rapid release of P32 as well as ultraviolet-absorbing material at 260 mμ, in the supernatant fluid.