A multiplex PCR for detection of genes encoding exfoliative toxins from Staphylococcus hyicus
A multiplex PCR for detection of genes encoding exfoliative toxins from Staphylococcus hyicus
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DOI：
10.1111/j.1365-2672.2004.02258.x
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发表时间：
2004-01-01
影响因子：
4
通讯作者：
Ahrens, P
中科院分区：
文献类型：
--
作者：
Andresen, LO;Ahrens, P
目的：目的建立多重PCR方法检测猪葡萄球菌（Staphylococcus hyicus）表皮脱落毒素ExhA、ExhB、ExhC和ExhD基因，以了解金黄色葡萄球菌（Staphylococcus hyicus）表皮脱落毒素的流行情况。方法和结果：采用编码四种不同剥脱性毒素的基因中的每一种的特异性引物的多重PCR被开发，并使用葡萄球菌的集合进行评估。具有已知毒素类型猪链球菌和许多其它葡萄球菌物种。总共314个葡萄球菌。采用多重PCR技术对60例EE猪分离株进行了检测，结果显示ExhA、ExhB、ExhC和ExhD的检出率分别为20%、33%、18%和22%。和葡萄球菌的流行更全面的图片。本研究的意义和影响：多重PCR可用于研究致炎性葡萄球菌的流行情况。hyicus阐明猪EE的流行病学。多重PCR目前用于选择葡萄球菌。用于生产自体疫苗的Hyicus分离物。
Aims: To develop a multiplex PCR for detection of genes encoding the exfoliative toxins ExhA, ExhB, ExhC and ExhD from Staphylococcus hyicus and to estimate the prevalence of exfoliative toxins among Staph. hyicus isolates from Danish pig herds with exudative epidermitis (EE).Methods and Results: A multiplex PCR employing specific primers for each of the genes encoding four different exfoliative toxins was developed and evaluated using a collection of Staph. hyicus with known toxin type and a number of other staphylococcal species. A total of 314 Staph. hyicus isolates from pigs with EE were screened by multiplex PCR and the combined results of the present and previous investigations showed that ExhA, ExhB, ExhC and ExhD was found in 20, 33, 18 and 22%, respectively, of 60 cases of EE investigated.Conclusions: This study has provided a new tool for detection of toxigenic Staph. hyicus and a more comprehensive picture of the prevalence of the Staph. hyicus exfoliative toxins in Danish pig herds.Significance and Impact of the Study: The multiplex PCR can be used in studies on the prevalence of toxigenic Staph. hyicus elucidating the epidemiology of EE in pigs. The multiplex PCR is currently being used for selection of Staph. hyicus isolates for production of autogenous vaccine.