Electrochemical Detection of ct-dsDNA on Nanomaterial-modified Carbon Based Electrodes
Electrochemical Detection of ct-dsDNA on Nanomaterial-modified Carbon Based Electrodes
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DOI：
10.2174/1573411014666180426165425
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发表时间：
2019-01-01
影响因子：
1.8
通讯作者：
Uslu, Bengi
中科院分区：
文献类型：
--
作者：
Bozal-Palabiyik, Burcin;Dogan-Topal, Burcu;Uslu, Bengi
背景：纳米材料在改善电化学传感系统性能方面具有重要作用。纳米材料独特的物理和化学性质使其在工程、材料和生物医学等领域的应用不断扩大。在过去的几年中，这些具有独特性能的材料在金属离子，蛋白质，生物标志物和药物化合物分析的实验方法设计中一直是首选。本文报道了两种不同的纳米材料的制备、表征及其在小牛胸腺DNA双链信号中的电化学应用。方法：采用介质阻挡放电等离子体处理多壁碳纳米管（MWCNTs-NH 2），使其表面带有胺基，并将其作为MWCNTs-NH 2/玻碳电极。此外，合成的介孔氧化硅MCM-41被化学胺功能化，并用作MCM-41-NH 2/碳糊电极。将小牛胸腺双链脱氧核糖核酸（ct-dsDNA）固定在修饰电极上，制备生物传感器。dsDNA/MCM-41-NH 2/碳糊生物传感器对鸟嘌呤信号具有选择性，而dsDNA/MWCNTs-NH 2/玻碳生物传感器对dsDNA信号具有电催化作用。采用伏安法研究了双链DNA修饰电极与抗癌药物依托泊苷（etoposide，ETP）在水溶液中的相互作用。在MWCNTs-NH 2修饰玻碳电极上，增加与ETP的作用时间，腺嘌呤峰电流被猝灭。结论：MCM-41-NH 2分子筛对鸟嘌呤的选择性氧化峰强度降低，当被测物质的信号电位与腺嘌呤相同时，可利用MCM-41-NH 2分子筛的选择性作用。
Background: Nanomaterials have a significant role in improving the performance of electrochemical sensing systems. Unique physical and chemical properties have extended the application of nanomaterials in the fields of engineering, materials and biomedical science. In the last few years, these materials with unique properties have been preferred in the design of experimental approaches for the analysis of metal ions, proteins, biomarkers and pharmaceutical compounds. This paper reports preparation, characterization of two different nanomaterials and their electrochemical application on double-stranded calf-thymus DNA signals.Methods: The multi-walled carbon nanotubes were functionalized with amine groups (MWCNTs-NH2) by employing the dielectric barrier discharge plasma treatment and then applied as MWCNTs-NH2/glassy carbon electrode. Moreover, the synthesized mesoporous silica MCM-41 was chemically amine functionalized and used as MCM-41-NH2/carbon paste electrode. For biosensor preparation, a thin layer of calf thymus double stranded deoxyribonucleic acid (ct-dsDNA) was immobilized over the modified electrodes.Results: The influence of dsDNA immobilized substrate was investigated based on the electrochemical signals. While dsDNA/MCM-41-NH2/carbon paste biosensor showed a selective effect for guanine signals, the dsDNA/MWCNTs-NH2/glassy carbon biosensor presented clectrocatalytic effect for dsDNA signals. Both dsDNA modified electrodes were employed to explore the interaction between the dsDNA and the anticancer drug etoposide (ETP) in aqueous solution through voltammetric techniques. By increasing the interaction time with ETP, the adenine peak current was quenched in the presence of MWCNTs-NH2 based glassy carbon electrode. Whereas, in the presence of MCM-41-NH2 based CP electrode, selective interaction with guanine occurred and oxidation peak intensity was diminished.Conclusion: The selective effect of MCM-41-NH2 can be used when the studied substances give a signal with the same potential of adenine.