The study of the ratio and distribution of Th17 cells and Tc17 cells in asthmatic patients and the mouse model
The study of the ratio and distribution of Th17 cells and Tc17 cells in asthmatic patients and the mouse model
复制标题
哮喘患者及小鼠模型中Th17细胞和Tc17细胞比例及分布的研究
DOI：
10.12932/ap0268.31.2.2013
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发表时间：
2013-06-01
影响因子：
5
通讯作者：
Xiong, Weining
中科院分区：
文献类型：
--
作者：
Li, Kaiyan;Wang, Zhengyun;Xiong, Weining
背景：产生CD 8（+）IL-17的T细胞，即Tc 17细胞，是否在哮喘中发挥作用尚未确定。本研究的目的是评估和比较哮喘患者外周血Th 17细胞和Tc 17细胞的频率。方法：采用流式细胞术检测哮喘患者外周血和海水脾脏中Th 17和Tc 17细胞的数量、比例和分布。采用免疫荧光双标法检测海水肺组织中Th 17和Tc 17细胞。结果：流式细胞仪检测结果显示哮喘患者外周血中Th 17细胞在CD 3 + T细胞中的比例高于健康对照组（P
Background: Whether CD8(+) IL-17-producing T cells, namely Tc17 cells, play a role in asthma has not been determined. The aim of this study was to evaluate and compare the frequency of peripheral blood Th17 cells and Tc17 cells in asthmatic patients. In addition, the number, ratio and distribution of Th17 cells and Tc17 cells in the lung tissue and splenocytes of asthmatic mice were also investigated.Methods: Th17 and Tc17 cells in the peripheral blood samples of asthmatic patients and in marine spleens were detected by flow cytometric analysis. Th17 and Tc17 cells in marine lung tissues were detected by double immunofluorescence stain. IL-17A levels in marine bronchoalveolar lavage were detected by ELISA.Results: The result of the flow cytometric analysis showed the percentage of Th17 cells among CD3+ T cell populations in patients with asthma was higher than that in healthy controls (P