Development of a serotyping enzyme-linked immunosorbent assay system based on recombinant truncated hantavirus nucleocapsid proteins for New World hantavirus infection
Development of a serotyping enzyme-linked immunosorbent assay system based on recombinant truncated hantavirus nucleocapsid proteins for New World hantavirus infection
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DOI：
10.1016/j.jviromet.2012.06.006
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发表时间：
2012-10-01
影响因子：
3.1
通讯作者：
Arikawa, Jiro
中科院分区：
文献类型：
--
作者：
Koma, Takaaki;Yoshimatsu, Kumiko;Arikawa, Jiro
根据汉坦病毒核衣壳蛋白（NP）内部可变区（约230-302个氨基酸）的氨基酸序列变异性，新世界汉坦病毒分为五组。 Sin Nombre 病毒 (SNV)、安第斯山脉病毒、黑溪运河病毒 (BCCV)。卡里萨尔病毒（CARV）和卡诺德尔加迪托病毒分别属于第1、2、3、4和5组。使用缺乏 99 个 N 末端氨基酸 (trNP100) 的 SNV、CARV 和 BCCV 重组截短 NP，通过酶联免疫吸附测定 (ELISA) 成功对患者和啮齿动物血清进行血清分型。 BCCV 的 trNP100 对异源血清表现出较低的反应性。相比之下，完整重组 NP 抗原同等地检测同源和异源抗体。该结果与之前的研究结果一起表明 trNP100 可以区分新世界汉坦病毒组 1、2、3 和 4 组的病毒感染。使用 trNP100 进行血清分型 ELISA 可用于人类和啮齿动物的流行病学监测。 (C) 2012 年爱思唯尔 BM。版权所有。
New World hantaviruses were divided into five groups based on the amino acid sequence variability of the internal variable region (around 230-302 amino acids) of hantavirus nucleocapsid protein (NP). Sin Nombre virus (SNV), Andes virus, Black Creek Canal virus (BCCV). Carrizal virus (CARV) and Cano Delgadito virus belong to groups 1, 2, 3,4 and 5, respectively. Patient and rodent sera were serotyped successfully by an enzyme-linked immunosorbent assay (ELISA) with recombinant truncated NP lacking 99 N-terminal amino acids (trNP100) of SNV, CARV and BCCV. The trNP100 of BCCV showed lower reactivity to heterologous sera. In contrast, whole recombinant NP antigens detected both homologous and heterologous antibodies equally. The results together with results of a previous study suggest that trNP100 can distinguish infections among viruses in groups 1, 2, 3 and 4 of New World hantaviruses. The serotyping ELISA with trNP100 is useful for epidemiological surveillance in humans and rodents. (C) 2012 Elsevier BM. All rights reserved.