Characteristics of the core protein of the aggregating proteoglycan from the Swarm rat chondrosarcoma
Characteristics of the core protein of the aggregating proteoglycan from the Swarm rat chondrosarcoma
复制标题
Swarm大鼠软骨肉瘤聚集蛋白多糖核心蛋白的特征
DOI：
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发表时间：
1984
影响因子：
4
通讯作者：
B. Caterson
中科院分区：
文献类型：
--
作者：
J. Stevens;Y. Oike;C. Handley;V. Hascall;A. Hampton;B. Caterson
A ternary complex of hyaluronic acid‐binding region and link protein bound to hyaluronic acid was isolated from limit clostripain digests of proteoglycan aggregates isolated from the Swarm rat chondrosarcoma. Under these conditions, the hyaluronic acid‐binding region has a molecular weight of ≅ 65,000 (HA‐BR65). N‐terminal amino acids in the complex were selectively l4C‐carbamylated. The resulting derivatized HA‐BR65 was isolated, and tryptic peptide maps were prepared and developed on two‐dimensional TLC sheets. A single, labeled peptide was obtained which gave a Mr by ≅ 8,000 by SDS‐PAGE. Chymotrypsin digestion of the ternary complex reduced the molecular weight of HA‐BR65 to a polypeptide of ≅ 55,000 (HA‐BR55) which still retains the same N‐terminal tryptic peptide. Partial digestion of proteoglycan aggregates with clostripain generated a series of larger intermediates with the hyaluronic acid‐binding region. Direct SDS‐PAGE analysis revealed one major intermediate with Mr ≅ 109,000 (HA‐BR109) as well as HA‐BR65. After chondroitinase digestion, two additional prominent intermediates were observed on a SDS‐PAGE gel at Mr ≅ 120,000 (HA‐BR120) and ≅ 140,000 (HA‐BR140). All the intermediates were recognized by a monoclonal antibody specific for the hyaluronic acid‐binding region, and all of them contained the same N‐terminal tryptic peptide. The results indicate that the N terminus of the core protein is at the hyaluronic acid‐binding end of the proteoglycan and that the chondroitin sulfate chains are first present on the core protein in a region between 109,000 and 120,000 molecular weight away from the N terminus.
鼠群软骨肉瘤蛋白聚糖中硫酸软骨素的磷酸化。
DOI：
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发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
OegemaJr,TR;Kraft,EL;Jourdian,GW;VanValen,TR
通讯作者：
VanValen,TR