Potential in a single cancer cell to produce heterogeneous morphology, radiosensitivity and gene expression
Potential in a single cancer cell to produce heterogeneous morphology, radiosensitivity and gene expression
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DOI：
10.1269/jrr.46.43
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发表时间：
2005-03-01
影响因子：
2
通讯作者：
Imai, T
中科院分区：
文献类型：
--
作者：
Ban, S;Ishikawa, K;Imai, T
形态异质的集落是由一种人食管癌组织建立的培养细胞系（KYSE70）形成的。从 KYSE70 细胞的单个克隆（克隆 13）中分离出两个亚克隆。一个亚克隆（克隆13-3G）主要形成堆状集落，而另一个亚克隆（克隆13-6G）则形成扁平、扩散的集落。 X射线照射刺激细胞从堆积状态去分化为扁平、扩散状态。克隆 13-6G 细胞比其他 3 个细胞系对放射更敏感。通过寡核苷酸微阵列对基因表达水平进行聚类分析表明，在放射敏感性克隆13-6G细胞中，参与细胞粘附的基因表达上调，但参与对DNA损伤刺激反应的基因表达下调。数据表明，单个癌细胞有可能在形态、辐射敏感性和基因表达方面产生异质后代，并且辐射增强了癌细胞的去分化。
Morphologically heterogeneous colonies were formed from a cultured cell line (KYSE70) established from one human esophageal carcinoma tissue. Two subclones were separated from a single clone (clone 13) of KYSE70 cells. One subclone (clone 13-3G) formed mainly mounding colonies and the other (clone 13-6G) formed flat, diffusive colonies. X-irradiation stimulated the cells to dedifferentiate from the mounding state to the flat, diffusive state. Clone 13-6G cells were more radiosensitive than the other 3 cell lines. Clustering analysis for gene expression level by oligonucleotide microarray demonstrated that in the radiosensitive clone 13-6G cells, expression of genes involved in cell adhesion was upregulated, but genes involved in the response to DNA damage stimulus were downregulated. The data demonstrated that a single cancer cell had the potential to produce progeny heterogeneous in terms of morphology, radiation sensitivity and gene expression, and irradiation enhanced the dedifferentiation of cancer cells.