De-N-acetyllactotriaosylceramide as a novel cationic glycosphingolipid of bovine brain white matter: isolation and characterization.
De-N-acetyllactotriaosylceramide as a novel cationic glycosphingolipid of bovine brain white matter: isolation and characterization.
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脱-N-乙酰基乳三糖神经酰胺作为牛脑白质的新型阳离子鞘糖脂：分离和表征。
DOI：
10.1021/bi0504411
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Hakomori,Senitiroh
中科院分区：
文献类型：
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作者：
Hikita,Toshiyuki;Tadano-Aritomi,Keiko;Iida-Tanaka,Naoko;Ishizuka,Ineo;Hakomori,Senitiroh
以氯仿和甲醇为溶剂，经羧甲基葡聚糖凝胶和硅胶柱层析，从牛脑白色物质中分离得到一种新型阳离子脂质。其结构经质谱和核磁共振氢谱确证为去N-乙酰基乳糖三己糖神经酰胺（deNAcLc 3Cer）。通过用单克隆抗体5 F5进行薄层色谱免疫染色，检测白色物质提取物中该糖脂的天然存在，该单克隆抗体5 F5针对deNAcLc 3 Cer并识别具有游离NH 2基团的末端β-葡糖胺基（GlcNH 2）残基。以N-[14 C]乙酰基标记的Lc 3Cer为底物，在牛脑提取物中检测到一种能水解Lc 3Cer的N-乙酰基的脱N-乙酰基酶。对deNAcLc 3Cer的生物起源和可能的功能意义进行了讨论。
A novel cationic lipid was separated from bovine brain white matter by a series of chromatographies on carboxymethyl-Sephadex and silica gel in chloroform and methanol. Its structure was identified unambiguously as de-N-acetyllactotriaosylceramide (deNAcLc3Cer) by mass spectrometry and1H NMR. The natural occurrence of this glycolipid in white matter extract was detected by immunostaining of thin-layer chromatography with monoclonal antibody 5F5, which is directed to deNAcLc3Cer and recognizes the terminal β-glucosaminyl (GlcNH2) residue, having a free NH2group. A de-N-acetylase capable of hydrolyzing theN-acetyl group of Lc3Cer was detected in bovine brain extract using N-[14C]acetyl-labeled Lc3Cer as a substrate. The biogenesis and possible functional significance of deNAcLc3Cer are discussed.