Concerto Catalysis. Harmonising [NiFe] Hydrogenase and NiRu Model Catalysts
Concerto Catalysis. Harmonising [NiFe] Hydrogenase and NiRu Model Catalysts
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协奏曲催化。
DOI：
10.1039/b926061g
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
S. Ogo
中科院分区：
文献类型：
--
作者：
K. Ichikawa;K. Nonaka;T. Matsumoto,B. Kure;K-S. Yoon;Y. Higuchi;T. Yagi;S. Ogo
本文报道了天然[NiFe]氢化酶（Desulfovibrio vulgaris宫崎F）和我们以前报道的[NiRu]氢化酶模拟物的化学性质的成功合并。天然酶和模拟物的催化活性几乎相同，除了工作pH范围，这使我们能够在同一反应瓶中同时使用它们。以这种方式，D2和H2O之间的同位素交换可以在环境温度和压力下在温和条件下在一个锅中在扩展的pH范围（约2-10）内进行。
This communication reports the successful merging of the chemical properties of a natural [NiFe]hydrogenase (Desulfovibrio vulgaris Miyazaki F) and our previously reported [NiRu] hydrogenase-mimic. The catalytic activity of both the natural enzyme and the mimic is almost identical, with the exception of working pH ranges, and this allows us to use them simultaneously in the same reaction flask. In such a manner, isotope exchange between D2 and H2O could be conducted over an extended pH range (about 2–10) in one pot under mild conditions at ambient temperature and pressure.