Separation and quantification of choline and acetylcholine by thermospray liquid chromatography/mass spectrometry.
Separation and quantification of choline and acetylcholine by thermospray liquid chromatography/mass spectrometry.
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通过热喷雾液相色谱/质谱法分离和定量胆碱和乙酰胆碱。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
S. Weintraub
中科院分区：
文献类型：
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作者：
D. Liberato;A. Yergey;S. Weintraub
开发用于鉴定和确认生物来源分子的灵敏分析技术仍然是一个持续的挑战。通常，感兴趣的化合物存在于复杂的混合物中，并且在分析之前需要大量的样品制备。热喷雾液相色谱/质谱（LC/MS）已被证明是一种方法，它允许从复杂的混合物中分离目标分子和灵敏的特定检测使用质谱仪。在这项工作中，热喷雾LC/MS用于直接检测完整的，未衍生化的胆碱和乙酰胆碱从小鼠脑匀浆。我们的分析结果证实了以前的分析使用各种间接测量技术，从而表明，这种简单的直接分析具有广泛的潜在适用性。
The development of sensitive analytical techniques for use in the identification and qualification of molecules of biological origin remains a constant challenge. Often the compounds of interest are present in a complex mixture and require extensive sample preparation prior to analysis. Thermospray liquid chromatography/mass spectrometry (LC/MS) has been shown to be a method which allows for both separation of target molecules from complex mixtures and sensitive specific detection using a mass spectrometer. In this work thermospray LC/MS is used for the direct detection of intact, underivatized choline and acetylcholine from mouse brain homogenate. The results of our analysis corroborate previous analyses using a variety of indirect measurement techniques and thereby show that this simple direct analysis has wide potential applicability.