Insertional protein engineering for analytical molecular sensing.
Insertional protein engineering for analytical molecular sensing.
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DOI：
10.1186/1475-2859-5-15
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发表时间：
2006-04-03
影响因子：
6.4
通讯作者：
Villaverde A
中科院分区：
文献类型：
--
作者：
Ferraz RM;Vera A;Arís A;Villaverde A
复杂样品中低浓度分析物的定量检测在生物医学、食品和环境等领域成为一个迫切的需求。生物传感器是由生物受体和信号转换器组成的混合装置，由于生物分子识别的高度特异性，代表了非生物分析仪器的有价值的替代品。现有的蛋白质配体的广泛范围使这些大分子能够用作有效的受体，以覆盖多种应用。此外，适当的蛋白质工程方法能够进一步改善受体功能，例如增强配体结合的亲和力或特异性。最近，正在开发几种仅蛋白质的传感器，其中受体和信号转导子都是同一蛋白质的一部分，或者使用产生蛋白质的整个细胞作为转导子。在这两种情况下，由于不需要进一步的化学偶联，因此生产过程非常方便。然而，蛋白质平台是相当刚性的，限制了通过配体诱导的构象变化必然发生的适当信号转导。在这种情况下，插入蛋白质工程提供了可能性，开发新的设备，有效地响应配体相互作用的显着的构象变化，其中的特异性和幅度的传感响应可以调整到一个方便的水平，为特定的分析物物种。在本报告中，我们将讨论设计此类仪器所采取的主要工程方法以及由此产生的仅蛋白质生物传感器的相关示例。
The quantitative detection of low analyte concentrations in complex samples is becoming an urgent need in biomedical, food and environmental fields. Biosensors, being hybrid devices composed by a biological receptor and a signal transducer, represent valuable alternatives to non biological analytical instruments because of the high specificity of the biomolecular recognition. The vast range of existing protein ligands enable those macromolecules to be used as efficient receptors to cover a diversity of applications. In addition, appropriate protein engineering approaches enable further improvement of the receptor functioning such as enhancing affinity or specificity in the ligand binding. Recently, several protein-only sensors are being developed, in which either both the receptor and signal transducer are parts of the same protein, or that use the whole cell where the protein is produced as transducer. In both cases, as no further chemical coupling is required, the production process is very convenient. However, protein platforms, being rather rigid, restrict the proper signal transduction that necessarily occurs through ligand-induced conformational changes. In this context, insertional protein engineering offers the possibility to develop new devices, efficiently responding to ligand interaction by dramatic conformational changes, in which the specificity and magnitude of the sensing response can be adjusted up to a convenient level for specific analyte species. In this report we will discuss the major engineering approaches taken for the designing of such instruments as well as the relevant examples of resulting protein-only biosensors.
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影响因子：
4.8
作者：
Ferrer-Miralles, N;Feliu, JX;Villaverde, A
通讯作者：
Villaverde, A
影响因子：
5.6
作者：
Feliu, JX;Benito, A;Villaverde, A
通讯作者：
Villaverde, A
影响因子：
5.5
作者：
Coëffier, E;Clément, JM;Leclerc, C
通讯作者：
Leclerc, C
影响因子：
3.7
作者：
Cabana, M;Fernàndez, G;Martínez, MA
通讯作者：
Martínez, MA
影响因子：
3.9
作者：
Chiang, CF;Okou, DT;Williams, MNV
通讯作者：
Williams, MNV