Olfactory stimulation selectively modulates the OFF pathway in the retina of zebrafish.
Olfactory stimulation selectively modulates the OFF pathway in the retina of zebrafish.
复制标题
DOI：
10.1016/j.neuron.2013.05.001
复制
发表时间：
2013-07-10
期刊：
影响因子：
16.2
通讯作者：
Lagnado L
中科院分区：
文献类型：
--
作者：
Esposti F;Johnston J;Rosa JM;Leung KM;Lagnado L
嗅觉刺激对视觉表现的跨模态调节开始于视网膜，其中多巴胺能中间神经元接收来自嗅球的投射。然而，我们不知道嗅觉刺激如何改变视网膜内视觉信号的处理。我们通过在双极细胞末端表达SyGCaMP2和在神经节细胞中表达GCaMP3.5的转基因斑马鱼体内成像活性来研究这个问题。食物相关的氨基酸蛋氨酸减少了增益和增加的灵敏度的亮度和对比度的反应，通过关闭双极细胞，但不ON。嗅觉刺激的影响被阻断抑制多巴胺的摄取和释放。多巴胺受体的激活增加了体内突触传递的增益，并增强了分离的双极细胞中的突触钙电流。这些结果表明，嗅觉刺激通过突触前钙通道的多巴胺能调节来改变视网膜的敏感性，所述突触前钙通道控制通过OFF双极细胞的突触传递的增益。嗅觉刺激调节通过视网膜双极细胞的信号传递突触增益和敏感性的调节发生在OFF双极细胞而不是ON双极细胞多巴胺摄取抑制剂阻断气味诱导的突触增益变化多巴胺增强孤立双极细胞中的突触前钙通道Esposti et al.显示了嗅觉刺激选择性地调节斑马鱼视网膜OFF双极细胞突触传递。多巴胺通过作用于突触前钙通道在这种跨模态相互作用中起关键作用。
Cross-modal regulation of visual performance by olfactory stimuli begins in the retina, where dopaminergic interneurons receive projections from the olfactory bulb. However, we do not understand how olfactory stimuli alter the processing of visual signals within the retina. We investigated this question by in vivo imaging activity in transgenic zebrafish expressing SyGCaMP2 in bipolar cell terminals and GCaMP3.5 in ganglion cells. The food-related amino acid methionine reduced the gain and increased sensitivity of responses to luminance and contrast transmitted through OFF bipolar cells but not ON. The effects of olfactory stimulus were blocked by inhibiting dopamine uptake and release. Activation of dopamine receptors increased the gain of synaptic transmission in vivo and potentiated synaptic calcium currents in isolated bipolar cells. These results indicate that olfactory stimuli alter the sensitivity of the retina through the dopaminergic regulation of presynaptic calcium channels that control the gain of synaptic transmission through OFF bipolar cells. Olfactory stimuli regulate transmission of signals through retinal bipolar cells Modulation of synaptic gain and sensitivity occur in OFF bipolar cells but not ON An inhibitor of dopamine uptake blocks odor-induced changes in synaptic gain Dopamine potentiates presynaptic calcium channels in isolated bipolar cells Esposti et al. show that olfactory stimulation selectively modulates synaptic transmission from retinal OFF bipolar cells in zebrafish. Dopamine plays a key role in this cross modal interaction by acting on the presynaptic calcium channels.
登录
查看更多内容
影响因子：
21.1
作者：
Catterall, WA;Striessnig, J;Perez-Reyes, E
通讯作者：
Perez-Reyes, E
DOI：
10.1016/s1546-5098(06)25005-2
发表时间：
2007-01-01
期刊：
SENSORY SYSTEMS NEUROSCIENCE
影响因子：
--
作者：
Li, Lei;Maaswinkel, Hans
通讯作者：
Maaswinkel, Hans
影响因子：
5.3
作者：
IUVONE, PM;GAN, JW
通讯作者：
GAN, JW
影响因子：
5.5
作者：
Burrone, J;Lagnado, L
通讯作者：
Lagnado, L
影响因子：
4.4
作者：
LIMA, L;SCHMEER, C;URBINA, M
通讯作者：
URBINA, M