ISOLATION AND CHARACTERIZATION OF HISTONE-F1 IN RIBOSOMES
ISOLATION AND CHARACTERIZATION OF HISTONE-F1 IN RIBOSOMES
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DOI：
10.1016/0006-291x(70)91027-2
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发表时间：
1970-01-01
影响因子：
3.1
通讯作者：
ENGER, MD
中科院分区：
文献类型：
--
作者：
GURLEY, LR;WALTERS, RA;ENGER, MD
从纯化的胞质多聚核糖体和核糖体中分离出一种蛋白质，根据其电泳迁移率、提取性质、氨基酸分析和在聚丙烯酰胺凝胶中用茜素黑染色时的颜色，该蛋白质似乎是组蛋白fl。它存在于大小多聚核糖体、核糖体和核糖体亚基中。在用0.25 M NH4 Cl或0.01 M EDTA的0.2 M NaCl溶液处理后，它仍然附着在多聚核糖体上，这些溶液可除去非水溶性吸附的蛋白质。
A protein has been isolated from purified cytoplasmic polysomes and ribosomes which appears to be histone fl based on its electrophoretic mobility, extraction properties, amino acid analysis, and color when stained with alizarin black in polyacrylamide gels. It was found in large and small polysomes, ribosomes, and ribosomal subunits. It remained attached to polysomes after treatment with 0.25 M NH 4 C1 or with 0.01 M EDTA in 0.2 M NaCl, which remove extraneously adsorbed proteins.