RETROVIRAL RECOMBINATION AND REVERSE TRANSCRIPTION
RETROVIRAL RECOMBINATION AND REVERSE TRANSCRIPTION
复制标题
DOI：
10.1126/science.1700865
复制
发表时间：
1990-11-30
期刊：
影响因子：
56.9
通讯作者：
TEMIN, HM
中科院分区：
文献类型：
--
作者：
HU, WS;TEMIN, HM
逆转录病毒复制中重组发生率很高，其观察需要含有两种不同 RNA 分子（异二聚体颗粒）的病毒体。对单轮复制后形成的逆转录病毒重组体的分析表明，（i）非选择标记的变化比根据选择标记的重组率预期的更频繁； (ii)最初合成的负链强终止DNA的转移是分子内或分子间； (iii) 第一个合成的正链强终止 DNA 的转移始终是分子内的； (iv)负链强终止DNA的转移类型与观察到的模板转换数量之间存在很强的相关性。这些数据表明，逆转录病毒重组是有序的，并且发生在负链和正链 DNA 的合成过程中。
Recombination occurs at a high rate in retroviral replication, and its observation requires a virion containing two different RNA molecules (heterodimeric particles). Analysis of retroviral recombinants formed after a single round of replication revealed that (i) the nonselected markers changed more frequently than expected from the rate of recombination of selected markers; (ii) the transfer of the initially synthesized minus strand strong stop DNA was either intramolecular or intermolecular; (iii) the transfer of the first synthesized plus strand strong stop DNA was always intramolecular; and (iv) there was a strong correlation between the type of transfer of the minus strand strong stop DNA and the number of template switches observed. Thse data suggest that retroviral recombination is ordered and occurs during the synthesis of both minus and plus strand DNA.