Purification, antitumor and antioxidant activities in vitro of polysaccharides from the brown seaweed Sargassum pallidum
Purification, antitumor and antioxidant activities in vitro of polysaccharides from the brown seaweed Sargassum pallidum
复制标题
褐藻马尾藻多糖的纯化、体外抗肿瘤及抗氧化活性
DOI：
10.1016/j.foodchem.2008.04.012
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发表时间：
2008-11-15
期刊：
影响因子：
8.8
通讯作者：
Zeng, Xiaoxiong
中科院分区：
文献类型：
--
作者：
Ye, Hong;Wang, Keqi;Zeng, Xiaoxiong
Supercritical CO2 extraction, ultrasonic-aid extraction and membrane separation technology were applied to prepare Sargassum pallidum polysaccharides (SP). Three main fractions, SP-1, SP-2 and SP-3, were obtained by membranes of 1.0 X 10(-4) mm, pore size and normal molecular-weight cut-off of 50 kDa. The resulting three preparations were further purified by DEAE Cellulose-52 chromatography to afford seven polysaccharide fractions. Furthermore, the antitumor and antioxidant activities, in vitro, of the polysaccharide fractions were evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay and DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical-scavenging assay, respectively. SP-3-1 and SP-3-2 showed significantly higher antitumor activity against the HepG2 cells, A549 cells, and MGC-803 cells. SP-3 had the highest sulfate content (22.6%). These results indicate that the higher antitumor activity of SP-3-1 and SP-3-2 from SP-3 with lower molecular weights may be related to their molecular weights and sulfate contents. The antioxidant activities of SP-1, SP-2 and SP-3 were low at the tested concentration. (C) 2008 Elsevier Ltd. All rights reserved.
