Effect of substrate stiffness on proliferation and differentiation of periodontal ligament stem cells
Effect of substrate stiffness on proliferation and differentiation of periodontal ligament stem cells
复制标题
基底硬度对牙周膜干细胞增殖和分化的影响。
DOI：
10.1111/cpr.12478
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发表时间：
2018-10-01
影响因子：
8.5
通讯作者：
Cai, Xiaoxiao
中科院分区：
文献类型：
--
作者：
Liu, Nanxin;Zhou, Mi;Cai, Xiaoxiao
ObjectivesThe的目的是了解基板刚度（细胞外基质的机械因素）对牙周膜干细胞（PDLSCs）和其潜在mechanism.Materials和methodsElastic基板的效果制成混合2个组件，基地和固化剂的比例为10：1，20：1，30：1或40：1。用扫描电镜观察PDLSC形态。将PDLSC在不同弹性基质上培养后，评估细胞增殖和分化。数据进行了分析，使用单因素AN0VA.ResultsSEM显示在弹性基板上培养的PDLSCs的形态变化。PDLSC增殖随基质硬度增加而增加（P
ObjectivesThe aim of this study was to understand the effect of substrate stiffness (a mechanical factor of the extracellular matrix) on periodontal ligament stem cells (PDLSCs) and its underlying mechanism.Materials and methodsElastic substrates were fabricated by mixing 2 components, a base and curing agent in proportions of 10:1, 20:1, 30:1 or 40:1. PDLSC morphology was observed using scanning electron microscopy (SEM). Cell proliferation and differentiation were assessed after PDLSCs was cultured on various elastic substrates. Data were analysed using one-way ANOVA.ResultsSEM revealed variations in the morphology of PDLSCs cultured on elastic substrates. PDLSC proliferation increased with substrate stiffness (P