Feasibility of using a bone-targeted, macromolecular delivery system coupled with prostaglandin E(1) to promote bone formation in aged, estrogen-deficient rats.
Feasibility of using a bone-targeted, macromolecular delivery system coupled with prostaglandin E(1) to promote bone formation in aged, estrogen-deficient rats.
复制标题
使用骨针对骨的大分子递送系统以及前列腺素E（1）的可行性，以促进老年雌激素缺陷大鼠的骨形成。
DOI：
10.1007/s11095-008-9706-0
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发表时间：
2008-12
影响因子：
3.7
通讯作者：
Kopecek, J.
中科院分区：
文献类型：
--
作者：
Miller, S. C.;Pan, H.;Wang, D.;Bowman, B. M.;Kopeckova, P.;Kopecek, J.
Macromolecular delivery systems have therapeutic uses because of their ability to deliver and release drugs to specific tissues. The uptake and localization of HPMA copolymers using Asp8 as the bone-targeting moiety was determined in aged, ovariectomized (ovx) rats. PGE1 was attached via a cathepsin K-sensitive linkage to HPMA copolymer–Asp8 conjugate and was tested to determine if it could promote bone formation. The uptake of FITC-labeled HPMA copolymer–Asp8 conjugate (P-Asp8-FITC) on bone surfaces was compared with the mineralization marker, tetracycline. Then a targeted PGE1-HPMA copolymer conjugate (P-Asp8-FITC-PGE1) was given as a single injection and its effects on bone formation were measured 4 weeks later. P-Asp8-FITC preferentially deposited on resorption surfaces, unlike tetracycline. A single injection of P-Asp8-FITC-PGE1 resulted in greater indices of bone formation in aged, ovx rats. HPMA copolymers can be targeted to bone surfaces using Asp8, with preferential uptake on resorption surfaces. Additionally, PGE1 attached to the Asp8-targeted HPMA copolymers and given by a single injection resulted in greater bone formation measured 4 weeks later. This initial in vivo study suggests that macromolecular delivery systems targeted to bone may offer some therapeutic opportunities and advantages for the treatment of skeletal diseases.
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影响因子：
4.7
作者：
Wang, Dong;Miller, Scott C.;Kopecek, Jindrich
通讯作者：
Kopecek, Jindrich
影响因子：
4.5
作者：
Pan, Huaizhong;Kopeckova, Pavla;Kopecek, Jindrich
通讯作者：
Kopecek, Jindrich
影响因子：
6.2
作者：
Kasugai, S;Fujisawa, R;Ohya, K
通讯作者：
Ohya, K
影响因子：
14
作者：
RIHOVA, B;KOPECEK, J;MANCAL, P
通讯作者：
MANCAL, P
影响因子：
4.1
作者：
Mandelin, J;Hukkanen, M;Konttinen, YT
通讯作者：
Konttinen, YT