Properties of an alginate-gelatin-based bioink and its potential impact on cell migration, proliferation, and differentiation
Properties of an alginate-gelatin-based bioink and its potential impact on cell migration, proliferation, and differentiation
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基于藻酸盐-明胶的生物墨水的特性及其对细胞迁移、增殖和分化的潜在影响
DOI：
10.1016/j.ijbiomac.2019.06.017
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发表时间：
2019-08-15
影响因子：
8.2
通讯作者：
Huang, Sha
中科院分区：
文献类型：
--
作者：
Cheng, Liuhanghang;Yao, Bin;Huang, Sha
三维（3D）生物打印允许将细胞嵌入生物墨水中，创建基于细胞的3D结构以促进组织再生和修复。生物墨水应该与细胞生物相容，并且应该确定其对细胞行为的影响。藻酸盐-明胶（Alg-Gel）与小鼠足底真皮（PD）的混合物诱导表皮祖细胞用于汗腺再生，证实了3D支持在该过程中的作用。本研究旨在研究Alg-Gel-PD生物墨水的化学和物理性质，以及其对包埋的小鼠间充质干细胞（MSCs）的影响。MSC在具有基于Alg-凝胶的生物墨水提取物的2D培养物中显示出增加的增殖和迁移。基因表达分析证实MSC向汗腺细胞分化。该提取物对分化细胞中的蛋白质表达没有影响。将MSC与基于Alg-Gel的生物墨水混合用于3D生物打印，导致了分化特征的基因和蛋白质表达，包括YAP 1激活。生物墨水的机械强度与小鼠皮肤组织的机械强度相似，扫描电子显微镜显示PD诱导了更规则的孔结构，这表明包埋细胞的物理性质具有优势。这项研究确定了生物墨水对细胞行为的影响，从而通过3D细胞打印过程促进治疗性干细胞功能。(C)2019由Elsevier B. V.出版
Three-dimensional (3D) bioprinting allows embedding of cells within a bioink, creating cell-based 3D structures to promote tissue regeneration and repair. The bioink should be biocompatible with the cells and its effect on cell behavior should be determined. Alginate-gelatin (Alg-Gel) blends with mouse planta dermis (PD) induced epidermal progenitors for sweat gland regeneration, confirming the role of 3D support during the process. The present study aimed to investigate the chemical and physical properties of the Alg-Gel-PD bioink, and its effect on embedded mouse mesenchymal stem cells (MSCs). MSCs showed increased proliferation and migration in 2D culture with an Alg-Gel-based bioink extract. Gene expression analysis confirmed MSC differentiation towards sweat gland cells. The extract had no effect on protein expression in differentiated cells. Mixing MSCs with the Alg-Gel-based bioink for 3D bioprinting resulted in gene and protein expression characteristic of differentiation, including YAP1 activation. The mechanical strength of the bioink was similar to that of mouse dermal tissue and scanning electron microscopy showed that PD induced a more regular pore structure, suggesting advantages for the physical properties of the embedded cells. This study determined the influence of bioink on cellular behavior, thereby promoting therapeutic stem cell function via 3D cell printing processes. (C) 2019 Published by Elsevier B.V.