Polymorphism of bone morphogenetic protein 4 gene and its relationship with litter size of Jining Grey goats
Polymorphism of bone morphogenetic protein 4 gene and its relationship with litter size of Jining Grey goats
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济宁灰山羊骨形态发生蛋白4基因多态性及其与产羔数的关系
DOI：
10.1007/s11033-010-0556-6
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发表时间：
2011-10-01
影响因子：
2.8
通讯作者：
Li, K.
中科院分区：
文献类型：
--
作者：
Chu, M. X.;Lu, L.;Li, K.
设计两对引物（P1和P2），通过单链构象多态性检测高产力品种（济宁灰山羊）和低产力品种（波尔、安哥拉和内蒙古绒山羊）骨形态发生蛋白4（BMP4）基因外显子2和内含子2的单核苷酸多态性。结果表明，4个山羊品种的BMP4基因外显子2（引物P1）均未检测到多态性。对于内含子2（引物P2），在济宁灰山羊和内蒙古绒山羊中检测到3种基因型（AA、AB和BB），在安哥拉山羊中检测到2种基因型（AB和BB），在波尔山羊中仅检测到1种基因型（AA）。测序结果显示，与AA基因型相比，BB基因型的BMP4基因有一个突变(2203G>A)。济宁灰山羊AA、AB、BB基因型的产羔数差异不显着（P>0.05）。设计一对引物(P3),通过微卫星分析检测4个山羊品种BMP4基因3′侧翼区含有二核苷酸重复序列(CA)的多态性。对于引物 P3，在四个山羊品种中检测到了三种基因型（CC、CD 和 DD）。测序显示DD基因型比CC基因型多1个CA二核苷酸。 CC基因型的济宁灰狼比CD或DD基因型的济宁灰狼多产0.55个（P<0.05）或0.72个（P<0.05）。这些结果初步表明BMP4基因的等位基因C是提高山羊产羔数的潜在DNA标记。
Two pairs of primers (P1 and P2) were designed to detect single nucleotide polymorphisms of exon 2 and intron 2 of bone morphogenetic protein 4 (BMP4) gene in both high fecundity breed (Jining Grey goat) and low fecundity breeds (Boer, Angora and Inner Mongolia Cashmere goats) by single strand conformation polymorphism. Results showed that no polymorphism was detected for exon 2 (primer P1) ofBMP4gene in four goat breeds. For intron 2 (primer P2), three genotypes (AA, AB and BB) were detected in Jining Grey and Inner Mongolia Cashmere goats, two genotypes (AB and BB) in Angora goats, and only one genotype (AA) in Boer goats. Sequencing revealed one mutation (2203G>A) ofBMP4gene in the genotype BB in comparison to the genotype AA. The differences of litter size between AA, AB and BB genotypes were not significant (P> 0.05) in Jining Grey goats. A pair of primer (P3) was designed to detect polymorphism in the 3′ flanking region ofBMP4gene that contained dinucleotide repeated sequence (CA) in the four goat breeds by microsatellite analysis. For primer P3, three genotypes (CC, CD and DD) were detected in four goat breeds. Sequencing revealed one more CA dinucleotide in genotype DD than in genotype CC. The Jining Grey does with genotype CC had 0.55 (P< 0.05) or 0.72 (P< 0.05) kids more than those with genotype CD or DD. These results preliminarily indicated that allele C ofBMP4gene is a potential DNA marker for improving litter size in goats.