Reductive dechlorination of 2-chlorophenol by Anaeromyxobacter dehalogenans with an electrode serving as the electron donor
Reductive dechlorination of 2-chlorophenol by Anaeromyxobacter dehalogenans with an electrode serving as the electron donor
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DOI：
10.1111/j.1758-2229.2009.00118.x
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发表时间：
2010-04-01
影响因子：
3.3
通讯作者：
Lovley, Derek R.
中科院分区：
文献类型：
--
作者：
Strycharz, Sarah M.;Gannon, Sarah M.;Lovley, Derek R.
电极电位足够低，可以作为微生物呼吸的电子供体，但又足够高，可以避免氢的产生，已经被提出作为使用可溶性电子供体的替代方案，以刺激氯化污染物和/或金属的生物修复。然而，使用纯微生物培养物的这种呼吸形式仅在地杆菌物种中有报道。为了进一步评估这种生物修复策略，研究人员用去卤厌氧菌进行了研究，该厌氧菌以前曾报道过以乙酸为电子供体将2-氯酚还原脱氯为苯酚。脱卤厌氧杆菌可以通过电子转移到处于正电位的石墨电极上氧化醋酸盐，表明其与电极直接交换电子的能力。与标准氢电极相比，附着在-300 mV电极上的去卤厌氧杆菌将2-氯苯酚还原为苯酚。在没有去卤弧菌的情况下，没有脱氯反应，当电极的电子供应中断时，电极驱动的脱氯反应停止。除地杆菌以外的微生物可以接受电极上的电子进行厌氧呼吸，氯化芳香族化合物可以通过这种方式脱氯，这些发现表明，利用微生物-电极相互作用处理多种污染物可能具有很大的潜力。
Electrodes poised at potentials low enough to serve as an electron donor for microbial respiration, but high enough to avoid the production of hydrogen, have been proposed as an alternative to the use of soluble electron donors for stimulating the bioremediation of chlorinated contaminants and/or metals. However, this form of respiration using pure cultures of microorganisms has only been reported in Geobacter species. To further evaluate this bioremediation strategy studies were conducted with Anaeromyxobacter dehalogenans, which has previously been reported to reductively dechlorinate 2-chlorophenol to phenol with acetate as the electron donor. Anaeromyxobacter dehalogenans could oxidize acetate with electron transfer to a graphite electrode poised at a positive potential, demonstrating its ability to directly exchange electrons with electrodes. Anaeromyxobacter dehalogenans attached to electrodes poised at -300 mV versus standard hydrogen electrode reductively dechlorinated 2-chlorophenol to phenol. There was no dechlorination in the absence of A. dehalogenans and electrode-driven dechlorination stopped when the supply of electrons to the electrode was disrupted. The findings that microorganisms other than Geobacter species can accept electrons from electrodes for anaerobic respiration and that chlorinated aromatic compounds can be dechlorinated in this manner suggest that there may be substantial potential for treating a diversity of contaminants with microbe-electrode interactions.