Neutrophil kinetics in chronic neutropenia.
Neutrophil kinetics in chronic neutropenia.
复制标题
慢性中性粒细胞减少症的中性粒细胞动力学。
DOI：
10.1182/blood.v54.3.581.bloodjournal543581
复制
发表时间：
1979
期刊：
影响因子：
20.3
通讯作者：
C. Finch
中科院分区：
文献类型：
--
作者：
T. Price;M. Lee;D. Dale;C. Finch
对16例无脾肿大的慢性中性粒细胞减少症患者进行了骨髓中性粒细胞池大小和产生率以及血液中性粒细胞动力学的定量研究。骨髓中性粒细胞的构成由骨髓正常成红细胞的铁动力学估计和由骨髓切片确定的嗜中性粒细胞-红细胞比率确定。有丝分裂后池周转率来自有丝分裂后池大小和转运时间，后者由3 H-胸苷中性粒细胞出现时间确定。用32 P-二异丙基氟磷酸标记的自体中性粒细胞研究血液中性粒细胞动力学。16例患者中有12例的有丝分裂池大小为基底或低于基底。有丝分裂后中性粒细胞的周转率为6例亚基底，7例基底，3例高于基底。8例患者的血液中性粒细胞转换率在正常范围内，8例降低。无效粒细胞生成的程度通过比较有丝分裂池的相对大小、有丝分裂后池周转和血液周转来评估。在此基础上，16例患者中有13例表现出明显程度的无效粒细胞生成。无效的中性粒细胞生产发生在中性粒细胞发育的早期和晚期。这些研究表明，大多数患有慢性中性粒细胞减少症而无脾肿大的患者缺乏对中性粒细胞减少症的增殖性骨髓反应，并表明无效的粒细胞生成是这种疾病的共同特征。
Quantitative studies of bone marrow neutrophil pool sizes and production rates and of blood neutrophil kinetics were performed in 16 patients with chronic neutropenia without splenomegaly. Marrow netrophil cellularity was determined from a ferrokinetic estimate of marrow normoblasts and from neutrophil-erythroid ratios determined from marrow sections. Postmitotic pool turnover was derived from the postmitotic pool size and transit time, the latter determined from 3H-thymidine neutrophil emergence time. Blood neutrophil kinetics were studied with 32P-diisopropylfluoophosphate-labeled autologous neutrophils. Mitotic pool size was basal or below basal in 12 of the 16 patients. The turnover of the post-mitotic neutrophils was subbasal in 6, basal in 7, and above basal in 3 patients. Blood neutrophil turnover was within the normal range in 8 patients and decreased in 8. The degree of ineffective granulocytopoiesis was assessed by comparing the relative size of the mitotic pool, postmitotic pool turnover, and blood turnover. On this basis, 13 of the 16 patients showed appreciable degrees of ineffective granulocytopoiesis. Ineffective neutrophil production occurred both early and late in neutrophil development. These studies indicate that most patients with chronic neutropenia without splenomegaly lack a proliferative marrow response to the neutropenia and suggest that ineffective granulocytopoiesis is a common feature of this disorder.