Nanomaterial-modulated cellular sodium extrusion and vacuolar sequestration for salt tolerance
Nanomaterial-modulated cellular sodium extrusion and vacuolar sequestration for salt tolerance
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纳米材料调节细胞钠挤出和液泡隔离以提高耐盐性
DOI：
10.1039/d2en00623e
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发表时间：
2022-09-08
影响因子：
7.3
通讯作者：
Wang, Zhenyu
中科院分区：
文献类型：
--
作者：
Chen, Feiran;Zhu, Liqi;Wang, Zhenyu
纳米材料可以诱导植物对非生物环境胁迫的耐受，但其传感机制及其在细胞水平上的反应还有待进一步探讨。亮黄色2号烟草(Nictiana tabacum L.)用100mMNaC l攻击悬浮培养细胞，暴露于不同剂量(0.05、0.1、0.5 mg L-1)的纳米CeO_2(nCeO(2))或氮掺杂碳点(N-Cd)。NCeO(2)0.1 mg L-1或N-Cd(0.5 mg L-1)对缓解盐胁迫效果最好，且nCeO(2)优于N-Cd。值得注意的是，nCeO(2)处理4h后，Na+-GIPC感知-Na+排出相关基因(IPUT1、SOS3、SOS2和SOS1)的表达增强(5.3倍)，而编码Na+空泡隔离的NHX1基因的表达在NCeO(2)处理6h后被诱导了7.8倍，随后，nCeO(2)处理后，NCeO(2)对Na+-GIPC感知-Na+外流的净内流和Na+外流分别有293.6%和191.3%的强烈促进作用，而N-Cd没有明显的Na+外流，但观察到瞬时K+内流(3.0倍)。下游脂肪酸、氨基酸和碳水化合物的代谢修饰有利于植物对盐胁迫的适应。这项工作揭示了特定的纳米材料诱导的早期细胞感知和信号级联，为基于纳米材料的农业实践抗击盐胁迫做出了贡献。
Nanomaterials can induce plant tolerance to abiotic environmental stresses, whereas the sensing mechanism and the resulting response at the cellular level need further exploration. Bright Yellow 2 tobacco (Nicotiana tabacum L.) suspension-cultured cells challenged with 100 mM NaCl were exposed to increasing doses (0.05, 0.1, and 0.5 mg L-1) of nano-CeO2 (nCeO(2)) or nitrogen-doped carbon dots (N-CDs). nCeO(2) at 0.1 mg L-1 or N-CDs at 0.5 mg L-1 were optimal for alleviating salt stress and nCeO(2) was superior to N-CDs. Notably, the expression of genes (IPUT1, SOS3, SOS2, and SOS1) involved in Na+-GIPC perception-Na+-extrusion was stimulated (5.3-fold) after nCeO(2) exposure for 4 h, and NHX1 encoding Na+ vacuolar sequestration was induced by 7.8-fold after N-CDs treatment for 6 h. Subsequently, the net Ca2+ influx and Na+ efflux were strongly promoted by 293.6% and 191.3% after incubation with nCeO(2), while no obvious Na+ extrusion but the transient K+ influx (by 3.0-fold) was observed for N-CDs. The downstream metabolic modifications in fatty acids, or amino acids and carbohydrates could favor the adaptation to salt stress. This work reveals the specific nanomaterial-induced early cell perception and signaling cascade, contributing to the nano-based agricultural practices for combating salt stress.