Modular platform for low-light microscopy
Modular platform for low-light microscopy
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DOI：
10.1364/boe.6.004585
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发表时间：
2015-11-01
影响因子：
3.4
通讯作者：
Pratx, Guillem
中科院分区：
文献类型：
--
作者：
Kim, Tae Jin;Tuerkcan, Silvan;Pratx, Guillem
使用生物发光、放射发光和低激发荧光等低光技术的细胞成像受到越来越多的关注，特别是由于高灵敏度检测器的广泛商业化。然而，微弱的信号仍然被认为难以使用传统显微镜成像，市场上唯一的低光显微镜主要针对生物发光成像进行了优化。在这里，我们开发了一种新型模块化显微镜，该显微镜具有成本效益，适合对不同的低光发光模式进行成像。结果表明，该显微镜系统具有出色的像差校正能力和增强的图像分辨率，捕获生物发光、放射发光和落射荧光图像并与商用生物发光显微镜进行比较。 (C) 2015年美国光学学会
Cell imaging using low-light techniques such as bioluminescence, radioluminescence, and low-excitation fluorescence has received increased attention, particularly due to broad commercialization of highly sensitive detectors. However, the dim signals are still regarded as difficult to image using conventional microscopes, where the only low-light microscope in the market is primarily optimized for bioluminescence imaging. Here, we developed a novel modular microscope that is cost-effective and suitable for imaging different low-light luminescence modes. Results show that this microscope system features excellent aberration correction capabilities and enhanced image resolution, where bioluminescence, radioluminescence and epifluorescence images were captured and compared with the commercial bioluminescence microscope. (C) 2015 Optical Society of America