Metabolic Capability of a Predominant Halanaerobium sp in Hydraulically Fractured Gas Wells and Its Implication in Pipeline Corrosion
Metabolic Capability of a Predominant Halanaerobium sp in Hydraulically Fractured Gas Wells and Its Implication in Pipeline Corrosion
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DOI：
10.3389/fmicb.2016.00988
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发表时间：
2016-06-22
影响因子：
5.2
通讯作者：
Suflita, Joseph M.
中科院分区：
文献类型：
--
作者：
Liang, Renxing;Davidova, Irene A.;Suflita, Joseph M.
与来自水力压裂操作的采出水相关的微生物活动可导致气体酸化和碳钢设备的腐蚀。我们研究了采出水的微生物生态学以及巴内特页岩气田中普遍存在的微生物在腐蚀中的潜在作用。微生物群落主要由嗜盐、产硫菌组成，反映了含硫物种（S2 O32-、SO 42-和HS-）的高盐水的地球化学条件。随后分离出一种主要的嗜盐细菌（菌株DL-01），并鉴定为属于嗜盐菌属。分离物可以降解瓜尔胶，一种用于压裂液中的多糖聚合物，产生乙酸盐和硫化物在10%NaCl介质中，在37 ℃时，硫代硫酸盐可用。为了减轻硫化物和乙酸盐的潜在有害影响，发现季铵化合物相对于戊二醛和四（羟甲基）硫酸鳞是抑制菌株DL-01的生长和代谢活性的有效杀生物剂。总的来说，我们的研究结果表明，与非常规页岩气开采相关的主要嗜盐菌可能会增殖，并从水力压裂液中使用的多糖的代谢中产生硫化物和乙酸盐。这些代谢产物可能会返回到地表并通过管道运输，从而导致下游基础设施的点蚀。
Microbial activity associated with produced water from hydraulic fracturing operations can lead to gas souring and corrosion of carbon-steel equipment. We examined the microbial ecology of produced water and the prospective role of the prevalent microorganisms in corrosion in a gas production field in the Barnett Shale. The microbial community was mainly composed of halophilic, sulfidogenic bacteria within the order Halanaerobiales, which reflected the geochemical conditions of highly saline water containing sulfur species (S2O32-, SO42-, and HS-). A predominant, halophilic bacterium (strain DL-01) was subsequently isolated and identified as belonging to the genus Halanaerobium. The isolate could degrade guar gum, a polysaccharide polymer used in fracture fluids, to produce acetate and sulfide in a 10% NaCl medium at 37 degrees C when thiosulfate was available. To mitigate potential deleterious effects of sulfide and acetate, a quaternary ammonium compound was found to be an efficient biocide in inhibiting the growth and metabolic activity of strain DL-01 relative to glutaraldehyde and tetrakis (hydroxymethyl) phosphonium sulfate. Collectively, our findings suggest that predominant halophiles associated with unconventional shale gas extraction could proliferate and produce sulfide and acetate from the metabolism of polysaccharides used in hydraulic fracturing fluids. These metabolic products might be returned to the surface and transported in pipelines to cause pitting corrosion in downstream infrastructure.