MicroRNA-encoded behavior in Drosophila.
MicroRNA-encoded behavior in Drosophila.
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DOI：
10.1126/science.aad0217
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发表时间：
2015-11-13
期刊：
影响因子：
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通讯作者：
Alonso CR
中科院分区：
文献类型：
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作者：
Picao-Osorio J;Johnston J;Landgraf M;Berni J;Alonso CR
microRNA调控与行为规范之间的关系才刚刚开始被探索。在这里，我们发现果蝇幼虫中单个microRNA位点（miR-iab4/8）的突变会影响动物在颠倒（自我纠正）时纠正其方向的能力。参与这种行为的microRNA靶标之一是Hox基因Ultrabithorax，其在两个异聚神经元中的抑制会导致自我纠正缺陷。体内神经活动分析显示，这些神经元，即自扶正节点（SRN），在野生型和miRNA突变体中具有不同的活动模式，而热致操作SRN活性会导致自扶正行为的改变。因此，我们的工作揭示了微rna编码的行为，并表明其他微rna也可能参与果蝇和其他物种的行为控制。
The relationship between microRNA regulation and the specification of behaviour is only beginning to be explored. Here we find that mutation of a single microRNA locus (miR-iab4/8) in Drosophila larvae affects the animal’s capacity to correct its orientation if turned upside-down (self-righting). One of the microRNA targets involved in this behaviour is the Hox gene Ultrabithorax whose derepression in two metameric neurons leads to self-righting defects. In vivo neural activity analysis reveals that these neurons, the self-righting node (SRN), have different activity patterns in wild type and miRNA mutants whilst thermogenetic manipulation of SRN activity results in changes in self-righting behaviour. Our work thus reveals a microRNA-encoded behaviour and suggests that other microRNAs might also be involved in behavioural control in Drosophila and other species.