[Screening and detecting of proteins interacting with mPem].
[Screening and detecting of proteins interacting with mPem].
复制标题
[与mPem相互作用的蛋白质的筛选和检测]。
DOI：
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
Tian
中科院分区：
文献类型：
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作者：
Zhi;Fen Guo;Yue;Shiqian Li;Xin Zhang;Hong;Tian
mPem是一种同源盒基因，在小鼠个体发育过程中以特定的时间和阶段表达。Pem转录本在7天和8天的小鼠胚胎中丰富，但此后急剧减少。在第9天，它们在胎盘和卵黄囊中变得丰富，一直持续到分娩。虽然Pem转录本在大多数成人组织中检测不到，但它们存在于生殖系统中，如睾丸、附睾和卵巢。这表明Pem在胚胎发生和生殖发育过程中具有重要作用。为了研究mPem蛋白的功能，我们采用基于GAL4的酵母双杂交方法筛选含有mPem全长的7天小鼠胚胎文库。发现3种蛋白与mPem蛋白相互作用。其中之一就是Mdfic。我们证实了mPem和Mdfic在酵母和体外的相互作用。Mdfic，包含MyoD家族抑制域，编码MyoD家族抑制域（I-mfa域）。mPem和Mdfic之间的相互作用提示它们可能形成转录调控复合体来调控胚胎分化。
mPem, a homeobox gene, is expressed in a time and stage specific manner during murine ontogeny. Pem transcripts are abundant in 7- and 8-day mouse embryos, but decrease precipitously thereafter. On Day 9 they become abundant in placenta and yolk sac, persisting there until parturition. Although Pem transcripts are not detectable in most of adult tissues, they are present in reproductive system such as testis, epididymis and ovary. This indicates a important role for Pem during embryogenesis and reproductive development. To study the function of mPem protein, we used a GAL4 based yeast two-hybrid assay to screen a 7-day mouse embryo library with full-length of mPem. 3 proteins were found interacting with mPem protein. One of theses is Mdfic. We confirmed the interaction between mPem and Mdfic in yeast and in vitro. Mdfic, MyoD family inhibitor domain containing, encodes the myoD family inhibitor domain (I-mfa domain). The interaction between mPem and Mdfic suggested they maybe form the transcriptional regulator complex to regulate embryo differentiation.