Role of naringenin in protection against diabetic hyperalgesia and tactile allodynia in male Wistar rats
Role of naringenin in protection against diabetic hyperalgesia and tactile allodynia in male Wistar rats
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DOI：
10.1007/s13105-014-0369-5
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发表时间：
2014-12-01
影响因子：
3.4
通讯作者：
Fazeli, Farzaneh
中科院分区：
文献类型：
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作者：
Hasanein, Parisa;Fazeli, Farzaneh
痛觉过敏和异常性疼痛是疼痛性糖尿病神经病变的常见表现。柚皮素（Naringenin，NA）具有抗炎、镇痛、抗糖尿病等多种生物活性。我们研究了不同剂量（20，50和100 mg/kg）NA给药对链脲佐菌素（STZ）诱导的大鼠痛觉过敏和异常性疼痛的影响。动物接受盐水或NA（20、50和100 mg/kg，p.o.;每日一次）持续8周。通过甩尾（TF）和福尔马林试验评估痛觉过敏。Von Frey细丝用于触觉异常性疼痛评价。实验结束后，称量大鼠体重，测定血糖和超氧化物歧化酶。在上述测试中，糖尿病引起显著的痛觉过敏和异常性疼痛。NA 50和100 mg/kg逆转糖尿病大鼠的化学和热痛觉过敏。NA（50和100 mg/kg）治疗的糖尿病大鼠和普瑞巴林治疗的糖尿病动物之间的疼痛反应没有显着差异。与相应的对照组相比，NA 20 mg/kg给药未改变对照组和糖尿病组的疼痛相关行为。NA 50和100 mg/kg可恢复糖尿病大鼠的高血糖以及降低的超氧化物歧化酶（SOD）活性水平。与未治疗的糖尿病大鼠相比，治疗的糖尿病大鼠的体重显著增加。长期口服NA（50和100 mg/kg）可通过引起低血糖和增加抗氧化酶SOD水平来改善糖尿病神经病变的某些方面。因此，NA在临床研究中成为治疗糖尿病神经病变的良好候选药物。
Hyperalgesia and allodynia are among the common manifestations of painful diabetic neuropathy. Naringenin (NA) has some biological activities, including anti-inflammatory, analgesic, and antidiabetic effects. We investigated the effects of NA administration at different doses, 20, 50, and 100 mg/kg, on streptozotocin (STZ)-induced hyperalgesia and allodynia in rats. The animals received saline or NA (20, 50, and 100 mg/kg, p.o.; once daily) for 8 weeks. Hyperalgesia was assessed by tail flick (TF) and formalin tests. Von Frey filaments were used for tactile allodynia evaluation. At the end, all rats were weighed and underwent plasma glucose and superoxide dismutase measurement. Diabetes caused significant hyperalgesia and allodynia during the above tests. NA 50 and 100 mg/kg reversed chemical and thermal hyperalgesia in diabetic rats. There were no significant differences in pain responses between NA (50 and 100 mg/kg)-treated diabetic rats and pregabalin-treated diabetic animals. Administration of NA 20 mg/kg did not alter pain-related behaviors in control and diabetic groups compared to the respective control ones. NA 50 and 100 mg/kg restored hyperglycemia as well as the decreased levels of (superoxide dismutase) SOD activity in diabetic rats. The body weight of treated diabetic rats increased significantly compared to untreated diabetics. Prolonged oral administration of NA (50 and 100 mg/kg) ameliorated some aspects of diabetic neuropathy by causing hypoglycemia and increasing the levels of antioxidant enzyme SOD. Therefore, NA makes a good candidate for treatment of diabetic neuropathy in clinical studies.