GUS gene expression driven by a citrus promoter in transgenic tobacco and 'Valencia' sweet orange
GUS gene expression driven by a citrus promoter in transgenic tobacco and 'Valencia' sweet orange
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DOI：
10.1590/s0100-204x2006001100008
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发表时间：
2006-11
影响因子：
0.8
通讯作者：
F. A. Azevedo;F. M. Filho;E. Schinor;Luis Gustavo de Paoli;B. J. Mendes;R. Harakava;D. Gabriel;Richard F. Lee
中科院分区：
文献类型：
--
作者：
F. A. Azevedo;F. M. Filho;E. Schinor;Luis Gustavo de Paoli;B. J. Mendes;R. Harakava;D. Gabriel;Richard F. Lee
本研究的目的是利用柑橘苯丙氨酸解氨酶（PAL）基因启动子（CsPP）驱动GUS基因对烟草和“瓦伦西亚”甜橙进行转化。以农杆菌为载体，以csp - gus /2201为载体，将烟草和‘瓦伦西亚’甜橙外植体与农杆菌共培养。在植株转化和再生后，利用GUS染色进行组织化学分析，发现CsPP启动子在烟草木质部组织中优先而不是唯一地赋予基因表达。在烟草和柑橘CsPP转基因植株的叶柄区域也检测到较弱的GUS染色。
The objective of this work was the transformation of tobacco and 'Valencia' sweet orange with the GUS gene driven by the citrus phenylalanine ammonia-lyase (PAL) gene promoter (CsPP). Transformation was accomplished by co-cultivation of tobacco and 'Valencia' sweet orange explants with Agrobacterium tumefaciens containing the binary vector CsPP-GUS/2201. After plant transformation and regeneration, histochemical analyses using GUS staining revealed that CsPP promoter preferentially, but not exclusively, conferred gene expression in xylem tissues of tobacco. Weaker GUS staining was also detected throughout the petiole region in tobacco and citrus CsPP transgenic plants.