Development of a new cyano-bonded column for high-performance liquid chromatography.
Development of a new cyano-bonded column for high-performance liquid chromatography.
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开发用于高效液相色谱的新型氰基键合柱。
DOI：
10.1016/s0021-9673(99)00861-4
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
M. Ohira
中科院分区：
文献类型：
--
作者：
K. Okusa;H. Tanaka;M. Ohira
将氰丙基基团化学键合到硅胶载体上，研制了一种独特的高效液相色谱氰键合柱，并对其色谱性能进行了描述。在不同条件下制备了8种氰丙基键合硅胶。将这些填充材料填充到不锈钢柱中，在正相和反相分配模式下研究其色谱性能和耐久性。氰丙基键合硅胶（CN）柱的分离选择性和耐用性取决于制备条件。特别地，考察了硅胶表面上氰丙基的密度与碱性化合物的不可逆吸附之间的关系。此外，实验结果表明，封端导致CN柱的分离选择性和耐久性差。
A unique cyano-bonded column for high-performance liquid chromatography was developed by chemically bonding cyanopropyl groups to the silica gel support, and its chromatographic performance was described. Eight cyanopropyl-bonded silica gels were prepared under different conditions. These packing materials were packed into a stainless steel column, the chromatographic properties and durability of which were investigated in both normal- and reversed-phase partition modes. The separating selectivity and the durability of cyanopropyl-bonded silica (CN) columns were dependent on the preparation conditions. Particularly, the relationship between the density of cyanopropyl groups on the surface of the silica gel and the irreversible adsorption of basic compounds was examined. Also, the experimental results indicated that endcapping resulted in the poor separating selectivity and durability of the CN columns.