Complexity of EBV homologous DNA in continuous lymphoblastoid cell lines.
Complexity of EBV homologous DNA in continuous lymphoblastoid cell lines.
复制标题
连续类淋巴母细胞系中 EBV 同源 DNA 的复杂性。
DOI：
10.1016/0042-6822(76)90144-6
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发表时间：
1976
期刊：
影响因子：
3.7
通讯作者：
E. Kieff
中科院分区：
文献类型：
--
作者：
R. Pritchett;M. Pedersen;E. Kieff
本文用EB病毒（EBV）DNA与淋巴母细胞DNA杂交的方法，测定了11个传代培养数年的EB病毒感染的淋巴母细胞系中EBV同源DNA的复杂性。分析的五个细胞系不含与EBV复制相关的早期（EA）或病毒衣壳（VCA）抗原。另外两个细胞系含有EA但不含VCA。在7个VCA阴性细胞系中，那些含有一些早期抗原或其中早期抗原可以用IUDR诱导的细胞系中，每个二倍体细胞基因组中有56、48和25个拷贝的EBV DNA序列超过90%。即使在诱导后也不含EA的五个细胞系具有23、8、8、6和2个拷贝/细胞的EBV基因组。这些数据表明，有一个非允许的细胞中的EBV DNA的拷贝数和这些细胞表达EA的能力之间的相关性。5 EA和VCA阴性的非允许的细胞系中的三个包含的DNA同源性超过90%的序列的EBV DNA。另外两个nonpermissive细胞系，Namalwa和SKL，其中包含两个和八个拷贝，分别，一些EBV DNA序列的DNA杂交动力学，建议，但不能证明，这些细胞系可能含有不完整的病毒基因组。在大多数非允许性淋巴母细胞样细胞系中保留病毒DNA的完整复杂性可能与这些细胞中病毒基因组的相对大量拷贝有关。
The complexity of Epstein-Barr Virus (EBV) homologous DNA in 11 EBV-infected lymphoblastoid cell lines which have been passaged for several years in culture was determined by hybridization of lymphoblastoid cell DNA to DNA extracted from EBV purified from HR-1 cells and labeledin vitro. Five of the cell lines analyzed contained no early (EA) or viral capsid (VCA) antigens which have been associated with the replication of EBV. Two other cell lines contained EA but not VCA. Of the seven VCA-negative cell lines, those which contained some early antigen or in which early antigen could be induced with IUDR had 56, 48, and 25 copies per diploid cell genome of more than 90% of the sequences of EBV DNA. Five cell lines which did not contain EA even after induction had 23, 8, 8, 6, and 2 copies per cell of the EBV genome. The data indicate that there is a correlation between the number of copies of EBV DNA in nonpermissive cells and the ability of these cells to express EA.Three of the five EA and VCA negative nonpermissive cell lines contained DNA homologous to more than 90% of the sequences of EBV DNA. The kinetics of hybridization of the DNA of two other nonpermissive cell lines, Namalwa and SKL, which contain two and eight copies, respectively, of some EBV DNA sequences, suggest but do not prove that these cell lines may contain incomplete viral genomes. The retention of the full complexity of viral DNA in most nonpermissive lymphoblastoid cell lines may be related to the relatively large number of copies of the viral genome in these cells.