What restricts the activity of mariner-like transposable elements?
What restricts the activity of mariner-like transposable elements?
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DOI：
10.1016/s0168-9525(97)01087-1
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发表时间：
1997-05-01
影响因子：
11.4
通讯作者：
Lohe, AR
中科院分区：
文献类型：
--
作者：
Hartl, DL;Lozovskaya, ER;Lohe, AR
最近描述了一些机制，这些机制可能对限制自然种群中水手样转座子(MLEs)的活性水平起到重要作用。这些机制包括生产过剩抑制，即增加转座酶的剂量会降低净活性。另一种机制是通过某些错义突变介导的，其中突变的转座酶蛋白在杂合突变/非突变基因型中削弱野生型转座酶的活性。另一种机制是通过保留转座酶结合活性的缺陷元件进行转座酶滴定的可能性。MLEs的调控问题不仅对理解MLEs的分子和进化遗传学具有重要的理论意义，而且在研究如何最好地利用MLEs进行害虫和病媒生物的种系转化方面也具有实际意义。
A number of mechanisms have recently been described that might be important in restricting the level of activity of mariner-like transposable elements (MLEs) in natural populations. These mechanisms include overproduction inhibition, in which increasing the dose of transposase decreases net activity. Another mechanism is mediated by certain missense mutations, in which a mutant transposase protein impairs the activity of the wild-type transposase in heterozygous mutant/nonmutant genotypes. A further mechanism is the potential for transposase titration by defective elements that retain transposase binding activity. The issue of regulation is not only of theoretical importance in understanding the molecular and evolutionary genetics of MLEs, but also of practical significance in learning how best to use MLEs in the germline transformation of insect pests and disease vectors.