(18)F-FDG PET imaging of murine atherosclerosis: association with gene expression of key molecular markers.
(18)F-FDG PET imaging of murine atherosclerosis: association with gene expression of key molecular markers.
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DOI：
10.1371/journal.pone.0050908
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Kjaer A
中科院分区：
文献类型：
--
作者：
Hag AM;Pedersen SF;Christoffersen C;Binderup T;Jensen MM;Jørgensen JT;Skovgaard D;Ripa RS;Kjaer A
通过检查 apoE−/− 小鼠 18F-FDG 摄取与动脉粥样硬化关键分子标志物基因表达之间的相关性，研究 18F-FDG 是否可用于动脉粥样硬化形成的体内成像。九组 apoE−/− 小鼠被给予正常食物或高脂肪饮食。在不同时间点，在专用动物扫描仪上进行18F-FDG PET/增强CT扫描。扫描后，对动物实施安乐死，切除主动脉，进行伽玛计数，从组织中提取RNA，并检测化疗（C-X-C基序）配体1（CXCL-1）、单核细胞趋化蛋白（MCP）-1、血管细胞粘附分子（VCAM）-1、分化分子簇（CD）-68、骨桥蛋白（OPN）、凝集素样氧化LDL受体的基因表达通过qPCR测量(LOX)-1、缺氧诱导因子(HIF)-1α、HIF-2α、血管内皮生长因子A(VEGF)和组织因子(TF)。通过 PET 和离体伽玛计数测量，接受高脂肪饮食的小鼠组中 18F-FDG 的摄取随着时间的推移而增加。所有检查的动脉粥样硬化标志物的基因表达与 18F-FDG 摄取显着相关。 TF 和 CD68 的相关性最强 (p<0.001)。多变量分析显示 CD68、OPN、TF 和 VCAM-1 是 18F-FDG 摄取的最重要贡献者。它们共同可以解释 60% 的 18F-FDG 吸收。我们已经证明 18F-FDG 可用于追踪 apoE−/− 小鼠动脉粥样硬化的进展。代表对动脉粥样硬化重要的不同分子过程的十种分子标记的基因表达与 18F-FDG 的摄取相关。特别是，CD68、OPN、TF 和 VCAM-1 的基因表达是摄取的有力预测因子。
To study whether 18F-FDG can be used for in vivo imaging of atherogenesis by examining the correlation between 18F-FDG uptake and gene expression of key molecular markers of atherosclerosis in apoE−/− mice. Nine groups of apoE−/− mice were given normal chow or high-fat diet. At different time-points, 18F-FDG PET/contrast-enhanced CT scans were performed on dedicated animal scanners. After scans, animals were euthanized, aortas removed, gamma counted, RNA extracted from the tissue, and gene expression of chemo (C-X-C motif) ligand 1 (CXCL-1), monocyte chemoattractant protein (MCP)-1, vascular cell adhesion molecule (VCAM)-1, cluster of differentiation molecule (CD)-68, osteopontin (OPN), lectin-like oxidized LDL-receptor (LOX)-1, hypoxia-inducible factor (HIF)-1α, HIF-2α, vascular endothelial growth factor A (VEGF), and tissue factor (TF) was measured by means of qPCR. The uptake of 18F-FDG increased over time in the groups of mice receiving high-fat diet measured by PET and ex vivo gamma counting. The gene expression of all examined markers of atherosclerosis correlated significantly with 18F-FDG uptake. The strongest correlation was seen with TF and CD68 (p<0.001). A multivariate analysis showed CD68, OPN, TF, and VCAM-1 to be the most important contributors to the uptake of 18F-FDG. Together they could explain 60% of the 18F-FDG uptake. We have demonstrated that 18F-FDG can be used to follow the progression of atherosclerosis in apoE−/− mice. The gene expression of ten molecular markers representing different molecular processes important for atherosclerosis was shown to correlate with the uptake of 18F-FDG. Especially, the gene expressions of CD68, OPN, TF, and VCAM-1 were strong predictors for the uptake.
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影响因子：
20.1
作者：
Mehta, Jawahar L.;Sanada, Nobuhito;Sawamura, Tatsuya
通讯作者：
Sawamura, Tatsuya
DOI：
10.1161/01.atv.0000074878.29805.d0
发表时间：
2003-06-01
影响因子：
8.7
作者：
Matsui, Y;Rittling, SR;Uede, T
通讯作者：
Uede, T
影响因子：
10.8
作者：
Hu, Changping;Dandapat, Abhijit;Mehta, Jawahar L.
通讯作者：
Mehta, Jawahar L.
影响因子：
6
作者：
Boisvert, WA;Rose, DM;Terkeltaub, RA
通讯作者：
Terkeltaub, RA
影响因子：
3.5
作者：
Qi, JY;Leahy, RM;Farquhar, TH
通讯作者：
Farquhar, TH