MicroRNAs Expressed during Lung Cancer Development Are Expressed in Human Pseudoglandular Lung Embryogenesis
MicroRNAs Expressed during Lung Cancer Development Are Expressed in Human Pseudoglandular Lung Embryogenesis
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DOI：
10.1159/000201569
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发表时间：
2009-01-01
期刊：
影响因子：
3.5
通讯作者：
Monzo, Mariano
中科院分区：
文献类型：
--
作者：
Navarro, Alfons;Marrades, Ramon M.;Monzo, Mariano
巴塞尔背景/目的：微小RNA（miRNAs）在小鼠胚胎发育过程中发挥作用，在肿瘤发生中也很重要。为了研究是否有类似的模式的miRNA表达水平在假腺人胚肺和人肺肿瘤，我们已经分析了18个miRNA（let-7家族，miR-17-92簇，miR-221和miR-222）在人胚肺样本和配对的肺肿瘤和正常肺组织样本，并将结果与临床病理特征。方法：从12例人胚肺组织、33例肺肿瘤组织和33例配对正常肺组织中提取RNA。通过定量实时PCR评估miRNA。结果如下：let-7家族成员在胚胎肺组织和肺肿瘤中下调，miR-17-92簇和miR-221成员在胚胎肺组织和肺肿瘤中过表达。低水平let-7 c与无转移（p = 0.015）、早期非小细胞肺癌（NSCLC，p = 0.05）和吸烟者（p = 0.009）相关。高水平的miR-106 a与小细胞肺癌相关（p = 0.031），高水平的miR-19 a与晚期NSCLC相关（p = 0.008）。结论：我们的研究支持癌症模型作为正常发育的改变，因为许多miRNA在早期人类肺发育和肺癌发展的I-II期中表达相似。版权所有（C）2009 S. Karger AG，巴塞尔
Basel Background/Aims: MicroRNAs (miRNAs) play a role during mouse embryonic development and are also important in carcinogenesis. In order to investigate whether there are similar patterns of miRNA expression levels in pseudoglandular human embryonic lung and in human lung tumors, we have analyzed 18 miRNAs (the let-7 family, the miR-17-92 cluster, miR-221 and miR-222) in human embryonic lung samples and in paired lung tumor and normal lung tissue samples and correlated the results with clinicopathological characteristics. Methods: RNA was obtained from 12 human embryonic lung samples, 33 lung tumor samples and 33 paired normal lung samples. miRNAs were assessed by quantitative real-time PCR. Results: Members of the let-7 family were downregulated and members of the miR-17-92 cluster and miR-221 were overexpressed both in embryonic lung tissue and in lung tumors. Low levels of let-7c were associated with absence of metastases (p = 0.015), early-stage non-small cell lung cancer (NSCLC, p = 0.05), and smokers (p = 0.009). High levels of miR-106a were associated with small-cell lung cancer (p = 0.031), and high levels of miR-19a with advanced NSCLC (p = 0.008). Conclusion: Our study lends support to the model of cancer as an alteration of normal development, as many miRNAs were similarly expressed in early human lung development and stage I-II of lung cancer development. Copyright (C) 2009 S. Karger AG, Basel