Brain-derived neurotrophic factor regulates the maturation of layer 4 fast-spiking cells after the second postnatal week in the developing barrel cortex
Brain-derived neurotrophic factor regulates the maturation of layer 4 fast-spiking cells after the second postnatal week in the developing barrel cortex
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DOI：
10.1523/jneurosci.3345-06.2007
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发表时间：
2007-02-28
影响因子：
5.3
通讯作者：
Nakamura, Shun
中科院分区：
文献类型：
--
作者：
Itami, Chiaki;Kimura, Fumitaka;Nakamura, Shun
据报道，脑源性神经营养因子（BDNF）在调节感觉皮质发育的可塑性中起关键作用。在视觉皮层，涉及gaba能神经元的神经元回路的成熟已被证明触发了一个关键时期。迄今为止，已知几种gaba能神经元，每一类都被认为发挥着不同的功能。其中，含有小白蛋白（PV）的快速尖峰（FS）细胞被认为参与了关键时期的开始。在这里，我们报道BDNF在桶状皮质塑性期PV-FS细胞的正常发育中起重要作用。我们发现，PV-FS细胞的电生理特征，如低尖峰适应率，响应超极化的电压下降，在出生后第二周左右开始发展，并在几天内达到成体水平。我们还发现，在相同的发育时间后，也获得了对PV的免疫反应性。然后，使用BDNF（-/-）小鼠，我们发现这些电生理和化学性质不发达或根本没有出现。我们认为BDNF调节PV-FS细胞的电生理和免疫组织化学特性的发展。由于BDNF被认为调节可塑性的启动，我们的研究结果强烈表明BDNF通过促进PV-FS gabaergy神经元的功能发育参与了关键时期的调节。
yBrain-derived neurotrophic factor ( BDNF) has been reported to play a critical role in modulating plasticity in developing sensory cortices. In the visual cortex, maturation of neuronal circuits involving GABAergic neurons has been shown to trigger a critical period. To date, several classes of GABAergic neurons are known, each of which are thought to play distinct functions. Of these, parvalbumin (PV)containing, fast-spiking (FS) cells are suggested to be involved in the initiation of the critical period. Here, we report that BDNF plays an essential role in the normal development of PV-FS cells during a plastic period in the barrel cortex. We found that characteristic electrophysiological properties of PV-FS cells, such as low spike adaptation ratio, reduced voltage sags in response to hyperpolarization, started to develop around the second postnatal week and attained adult level in several days. We also found that immunoreactivity against PV was also acquired after the similar developmental time course. Then, using BDNF(-/-) mice, we found that these electrophysiological as well as chemical properties were underdeveloped or did not appear at all. We conclude BDNF regulates the development of electrophysiological and immunohistochemical characteristics in PV-FS cells. Because BDNF is suggested to regulate the initiation of plasticity, our results strongly indicate that BDNF is involved in the regulation of the critical period by promoting the functional development of PV-FS GABAergic neurons.