Molecular Characterization of the Sigma Class Gutathione S-Transferase From Chilo suppressalis and Expression Analysis Upon Bacterial and Insecticidal Challenge
Molecular Characterization of the Sigma Class Gutathione S-Transferase From Chilo suppressalis and Expression Analysis Upon Bacterial and Insecticidal Challenge
复制标题
二化螟 Sigma 类谷胱甘肽 S-转移酶的分子表征以及细菌和杀虫挑战下的表达分析
DOI：
10.1603/ec11181
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发表时间：
2011-12-01
影响因子：
2.2
通讯作者：
Ye, Gongyin
中科院分区：
文献类型：
--
作者：
Huang, Jia;Wu, Shunfan;Ye, Gongyin
昆虫谷胱甘肽s -转移酶（GSTs）在解毒过程中发挥重要作用，并与杀虫剂抗性有关。从亚洲水稻主要害虫之一的亚洲稻螟（Chilo suppressalis, Walker）中克隆了编码sigma类GST蛋白（CsGSTsigma）的全长cDNA序列。氨基酸序列比较表明，CsGSTsigma与家蚕（Bombyx mori (L.)）中分离的sigma GST高度相似。构建了CsGSTsigma的同源性模型，其结合谷胱甘肽的环境与果蝇（Drosophila melanogaster Meigen）中sigma GST的等效位点相同。研究了亚洲稻螟CsGSTsigma基因的相对mRNA表达量在发育过程中的变化，该基因在成虫阶段表达量最高，其次是3龄幼虫阶段。此外，一种革兰氏阳性细菌和两种化学杀虫剂均可诱导CsGSTsigma表达增加，提示CsGSTsigma可能作为抗氧化酶来对抗病原菌和外源物的负面影响。
The insect glutathione S-transferases (GSTs) play an important role in the detoxification of xenobiotic compounds and are related to insecticides resistance. The full-length cDNA sequences encoding the sigma class GST protein (CsGSTsigma) was cloned from the Asiatic rice borer, Chilo suppressalis (Walker), one of the most important rice pests in Asia. The comparison of amino acid sequences showed that CsGSTsigma is highly similar to the sigma GST isolated from the domestic silkworm, Bombyx mori (L.). A homology model of CsGSTsigma was constructed and its binding environment for GSH is identical to that in the equivalent site of sigma GST from the fruit fly, Drosophila melanogaster Meigen. The developmental changes of the relative mRNA expression levels of CsGSTsigma were examined in Asiatic rice borer, and the highest expression level of this gene is in adult followed by the third-instar larvae stage. Furthermore, one gram-positive bacterium and two chemical insecticides were found to be able to induce the increasing expression of CsGSTsigma, suggesting that CsGSTsigma might work as an antioxidant enzyme to against the negative effects caused by both pathogens and xenobiotics.