Dual-Gradient Capillary Electrochromatography by Varying the Mobile Phase Composition and Applied Voltage
Dual-Gradient Capillary Electrochromatography by Varying the Mobile Phase Composition and Applied Voltage
复制标题
通过改变流动相组成和施加电压的双梯度毛细管电色谱
DOI：
10.2116/analsci.24.735
复制
发表时间：
2008
影响因子：
1.6
通讯作者：
H. Ohtani
中科院分区：
文献类型：
--
作者：
T. Sakai;Hiroyuki Nakagawa;S. Kitagawa;H. Ohtani
双梯度毛细管电色谱（DG-CEC）的发展提供了上级性能方面的分离离子化的分析物，在这种方法中，洗脱液的组成和施加的电压是不同的分离过程中。至于洗脱液组成的梯度，pH的变化不仅用于控制电泳迁移率，还用于控制分析物的保留因子。双梯度法在提高分离度和缩短色谱周期方面是有效的。采用多级电压放大的DG-CEC在8 min内实现了14种邻苯二甲醛标记氨基酸的分离。与普通的单梯度洗脱液相比，双梯度洗脱液的分离效率提高，特别是对保留率高的氨基酸。
Dual-gradient capillary electrochromatography (DG-CEC) was developed to provide superior performance with regard to the separation of ionized analytes; in this method, both the eluent composition and the applied voltage are varied during the separation procedure. As for the gradient in the eluent composition, a shift in the pH is employed to control not only the electrophoretic mobility, but also the retention factor of the analytes. The dual-gradient method was shown to be effective in increasing the resolution and reducing the chromatographic period of ionized analytes. Fourteen kinds of o -phthalaldehyde labeled amino acids were separated within 8 min using DG-CEC with multistage enlargement in the applied voltage. The separation efficiency increased particularly for highly retained amino acids in the dual-gradient, as compared to those in the ordinary single-gradient for the eluent.
用于在磺化萘酰亚胺改性硅基硅胶上进行肽毛细管电色谱分析的缓冲液浓度的逐步梯度。
DOI：
--
发表时间：
2005
期刊：
Journal of Chromatography A, 1064
影响因子：
--
作者：
K.Ohyama;et al.
通讯作者：
et al.
影响因子：
7.4
作者：
Yuji Ueki;T. Umemura;Jinxiang Li;T. Odake;K. Tsunoda
通讯作者：
Yuji Ueki;T. Umemura;Jinxiang Li;T. Odake;K. Tsunoda